رسالة جامعية
Μελέτη ισχαιμίας - επαναιμάτωσης εγκεφάλου κατόπιν αποκλεισμού καρωτίδων ; Experimental study of ischemia / reperfusion - induced brain injury following carotid artery occlusion
| العنوان: | Μελέτη ισχαιμίας - επαναιμάτωσης εγκεφάλου κατόπιν αποκλεισμού καρωτίδων ; Experimental study of ischemia / reperfusion - induced brain injury following carotid artery occlusion |
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| المؤلفون: | Dimopoulos, Christos, Δημόπουλος, Χρίστος |
| بيانات النشر: | National and Kapodistrian University of Athens Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ) |
| سنة النشر: | 2019 |
| المجموعة: | National Archive of PhD Theses (National Documentation Centre Greece) |
| مصطلحات موضوعية: | Αγγειακό εγκεφαλικό επεισόδιο, Κυκλοσπορίνη, Ισχαιμία - υποξία εγκεφάλου, Βλάβη επαναιμάτωσης, S100B πρωτεΐνη, Βιοδείκτες εγκεφαλικής βλάβης, Brain Infarction, Cyclosporine, Cerebral hypoxia - ischemia, Reperfusion injury, S100 calcium binding protein beta subunit, Stroke biomarkers, Ιατρική και Επιστήμες Υγείας, Βασική Ιατρική, Κλινική Ιατρική, Medical and Health Sciences, Basic Medicine, Clinical Medicine |
| الوصف: | Background: Accumulating evidence has indicated that S100B protein may be involved in the pathophysiology of ischemia-reperfusion brain injury. Cyclosporine has been shown to have neuroprotective functions. This study investigated the effect of cyclosporine on S100B serum levels and the severity of brain tissue damage in a rat model of cerebral ischemia-reperfusion (I/R). Material/Methods: Twelve-week-old Wistar male rats were randomly divided into Control I/R and Cyclosporine I/R groups (n=10 each). Cyclosporine was given orally by gavage for 5 days prior to cerebral I/R, at a total volume of 15 mg/kg/day. Body weight was measured and all animals were subjected to 60-min focal ischemia by filament occlusion of the middle cerebral artery. ELISA was used to assess the concentrations of serum S100B and development of brain infarct size and neurological outcomes were determined at 2 and 24 h after occlusion withdrawal. Results: Cyclosporine improved the neurological deficit score and decreased the cerebral infarct size and body weight. S100B serum levels were significantly elevated in Cyclosporine-treated rats compared with untreated Control rats during the reperfusion phase. Total infarct size was positively associated with S100B serum levels in the Control I/R group, but no significant correlation was observed in the Cyclosporine I/R group. Conclusions: Cyclosporine seems to affect both ischemia-reperfusion brain tissue damage and S100B protein serum levels. S100B serum level appears to be a state marker for the severity of the cerebral ischemia-reperfusion, rather than a trait marker for Cyclosporine responsiveness. ; Εισαγωγή: Η πρωτεΐνη S100B εμπλέκεται στην παθοφυσιολογία του ισχαιμικού αγγειακού εγκεφαλικού επεισοδίου. Η παρούσα μελέτη διερεύνησε την επίδραση της κυκλοσπορίνης επί των επιπέδων S-100B ορού και τη σοβαρότητα της εγκεφαλικής βλάβης σε ένα πειραματικό μοντέλο ισχαιμίας-επαναιμάτωσης του εγκεφάλου. Υλικό & Μέθοδοι: Αρσενικοί επίμυες τύπου Wistar, ηλικίας δώδεκα εβδομάδων, χωρίστηκαν σε δύο ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | Greek, Modern (1453-) |
| العلاقة: | http://hdl.handle.net/10442/hedi/45283Test |
| DOI: | 10.12681/eadd/45283 |
| الإتاحة: | https://doi.org/10.12681/eadd/45283Test http://hdl.handle.net/10442/hedi/45283Test |
| رقم الانضمام: | edsbas.E8DCE368 |
| قاعدة البيانات: | BASE |
| DOI: | 10.12681/eadd/45283 |
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