رسالة جامعية
Λειτουργικές γονιδιωματικές προσεγγίσεις για τη μελέτη της μορφογένεσης στη Drosophila melanogaster ; Functional genomic approaches to study morphogenesis of Drosophila melanogaster
| العنوان: | Λειτουργικές γονιδιωματικές προσεγγίσεις για τη μελέτη της μορφογένεσης στη Drosophila melanogaster ; Functional genomic approaches to study morphogenesis of Drosophila melanogaster |
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| المؤلفون: | Μουρατίδου, Μαρία |
| بيانات النشر: | University of Patras Πανεπιστήμιο Πατρών |
| سنة النشر: | 2011 |
| المجموعة: | National Archive of PhD Theses (National Documentation Centre Greece) |
| مصطلحات موضوعية: | Δροσόφιλα, Τρασγελίνες, RNA παρεμβολή, Μελέτη σάρωσης, Μυϊκό σύστημα, Επιθήλιο φτερού, Μαλπιγγιανά σωληνάρια, Drosophila, Trasgelins, RNA interference, Screen, Muscle system, Wing epithelium, Malpighian tubules, CHD64, Φυσικές Επιστήμες, Βιολογία, Natural Sciences, Biological Sciences |
| الوصف: | In the past years many research groups have focused in the identification ofgenes that are involved in distinct biological processes and Drosophila has providedthe ideal model-organism due to the availability of several genetic tools. Theintroduction of RNAi technology has greatly facilitated the conduction of many largescale genetic analyses in Drosophila. Taking advantage of the available tools weconducted an RNAi-based screen for genes involved in the morphogenesis of thesomatic muscle system and wing epithelium of Drosophila melanogaster.Collectively, we tested 321 genes with either systemic or tissue-specific RNAisilencing in the mesoderm or a combination of both and discovered 58 novel geneswhich are required for proper development and homeostasis of the embryonic/larvalmuscular system. Surprisingly, in almost half of the cases we did not observe a lethalphenotype of complete penetrance arguing that the number of genes involved in theparticular process is greater than the one estimated based on the results of a recentlycompleted genome-wide scale RNAi-based muscle screen. In addition, we tested 242genes by tissue-specific gene inactivation in the epithelia and identified 32 that arerequired for adult viability and 24 that are indispensible for proper adult wingmorphogenesis. Among our positive hits we selected one that is required for bothprocesses for further examination, namely chd64.Chd64 encodes a protein that consists of a calponin-homology (CH) domainand a CLIK23 motif and exhibits 42% and 43% identity with the mammaliantransgelins 2 and 3 respectively. Transgelins comprise a highly conserved family ofproteins that have been implicated in the bundling and stabilization of actin filaments.The Drosophila genome bears three distinct loci: CG4694 or Mp20, CG14996 orChd64 and CG5023 by complete analogy to the mammalian genomes. Our initialresults showed that out of the three genes that code for the fly transgelins only chd64is required for viability and the proper development of muscle and epithelial ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | Greek, Modern (1453-) |
| العلاقة: | http://hdl.handle.net/10442/hedi/30351Test |
| DOI: | 10.12681/eadd/30351 |
| الإتاحة: | https://doi.org/10.12681/eadd/30351Test http://hdl.handle.net/10442/hedi/30351Test |
| رقم الانضمام: | edsbas.3740BA1C |
| قاعدة البيانات: | BASE |
| DOI: | 10.12681/eadd/30351 |
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