التفاصيل البيبلوغرافية
| العنوان: |
堆肥から分離したClostridium thermosaccharolyticum F - 9の β - グルコシダーゼ の性質 |
| المؤلفون: |
嶋田, 協, Shimada, Kyo, 袴田, 佳宏, Hakamada, Yoshihiro, 粟冠, 和郎, Sakka, Kazuo, 松嶋, 欽一, Matsushima, Kinichi |
| بيانات النشر: |
三重大学生物資源学部 |
| سنة النشر: |
1989 |
| المجموعة: |
MIUSE - Mie University Scholarly E-collection / 三重大学学術機関リポジトリ研究教育成果コレクション |
| مصطلحات موضوعية: |
thermophilic, β-glucosidase, thermostable enzyme |
| الوقت: |
463 |
| الوصف: |
application/pdf ; Nine strains of cellulolytic-thermophilic anaerobes were isolated from hot springs and composts and analyzed for their productivity of β-glucosidase (β-D-Glucoside glucohydrolase EC 3. 2. 1. 21). Among them, strain F-9 showed the highest β-glucosidase activity, and this strain was identified as a member of Clostridium thermosaccarolyticum on taxonomical characteristics. The intracellular β-glucosidase of the bacterium was purified 40-fold with a recovery of 6%. The purified enzyme preparation was chromatographically homogeneous. The molecular weight of about 350,000 was estimated by gel filtration using Sephadex G-150, Bio-Gel p-300 and Sepharose 4B. Its isoelectric point was estimated to be 4.6. The activity of the enzyme for cellobiose was very much lower than for p-nitrophenyl β-D-glucoside. The enzyme did not hydrolyse maltose, lactose, carboxy- methyl-cellulose and cellulose. It was strongly inhibited by D-glucono-δ-lactone and was sensitive to thiol reagents. ; departmental bulletin paper |
| نوع الوثيقة: |
other/unknown material |
| وصف الملف: |
application/pdf |
| اللغة: |
Japanese |
| تدمد: |
0915-0471 |
| العلاقة: |
三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University; 81; 84; AN10073846; https://mie-u.repo.nii.ac.jp/record/5690/files/AN100738460000208.pdfTest |
| الإتاحة: |
https://mie-u.repo.nii.ac.jp/record/5690/files/AN100738460000208.pdfTest |
| رقم الانضمام: |
edsbas.5D0B723F |
| قاعدة البيانات: |
BASE |
