التفاصيل البيبلوغرافية
| العنوان: |
Correcting for Naturally Occurring Mass Isotopologue Abundances in Stable-Isotope Tracing Experiments with PolyMID |
| المؤلفون: |
Heesoo Jeong, Yan Yu, Henrik J. Johansson, Frank C. Schroeder, Janne Lehtiö, Nathaniel M. Vacanti |
| المصدر: |
Metabolites; Volume 11; Issue 5; Pages: 310 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2021 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
stable-isotope tracing, mass isotopologue distribution, correction, metabolic flux analysis, natural abundances, metabolism |
| الوصف: |
Stable-isotope tracing is a method to measure intracellular metabolic pathway utilization by feeding a cellular system a stable-isotope-labeled tracer nutrient. The power of the method to resolve differential pathway utilization is derived from the enrichment of metabolites in heavy isotopes that are synthesized from the tracer nutrient. However, the readout is complicated by the presence of naturally occurring heavy isotopes that are not derived from the tracer nutrient. Herein we present an algorithm, and a tool that applies it (PolyMID-Correct, part of the PolyMID software package), to computationally remove the influence of naturally occurring heavy isotopes. The algorithm is applicable to stable-isotope tracing data collected on low- and high- mass resolution mass spectrometers. PolyMID-Correct is open source and available under an MIT license. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Endocrinology and Clinical Metabolic Research; https://dx.doi.org/10.3390/metabo11050310Test |
| DOI: |
10.3390/metabo11050310 |
| الإتاحة: |
https://doi.org/10.3390/metabo11050310Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.ED8BB6D9 |
| قاعدة البيانات: |
BASE |
