التفاصيل البيبلوغرافية
| العنوان: |
AT1 and AT2 Receptor Knockout Changed Osteonectin and Bone Density in Mice in Periodontal Inflammation Experimental Model |
| المؤلفون: |
Maria Laura de Souza Lima, Caroline Addison Carvalho Xavier de Medeiros, Gerlane Coelho Bernardo Guerra, Robson Santos, Michael Bader, Flavia Q. Pirih, Raimundo Fernandes de Araújo Júnior, Alan B. Chan, Luis J. Cruz, Gerly Anne de Castro Brito, Renata Ferreira de Carvalho Leitão, Ericka Janine Dantas da Silveira, Vinicius Barreto Garcia, Agnes Andrade Martins, Aurigena Antunes de Araújo |
| المصدر: |
International Journal of Molecular Sciences; Volume 22; Issue 10; Pages: 5217 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2021 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
bone, micro-computed tomography, periodontitis, inflammation, osteonectin |
| جغرافية الموضوع: |
agris |
| الوصف: |
Background: The aim of this study was to evaluate the role of AT1 and AT2 receptors in a periodontal inflammation experimental model. Methods: Periodontal inflammation was induced by LPS/Porphyromonas gingivalis. Maxillae, femur, and vertebra were scanned using Micro-CT. Maxillae were analyzed histopathologically, immunohistochemically, and by RT-PCR. Results: The vertebra showed decreased BMD in AT1 H compared with WT H (p < 0.05). The femur showed increased Tb.Sp for AT1 H and AT2 H, p < 0.01 and p < 0.05, respectively. The Tb.N was decreased in the vertebra (WT H-AT1 H: p < 0.05; WT H-AT2 H: p < 0.05) and in the femur (WT H-AT1 H: p < 0.01; WT H-AT2 H: p < 0.05). AT1 PD increased linear bone loss (p < 0.05) and decreased osteoblast cells (p < 0.05). RANKL immunostaining was intense for AT1 PD and WT PD (p < 0.001). OPG was intense in the WT H, WT PD, and AT2 PD when compared to AT1 PD (p < 0.001). AT1 PD showed weak immunostaining for osteocalcin compared with WT H, WT PD, and AT2 PD (p < 0.001). AT1 H showed significantly stronger immunostaining for osteonectin in fibroblasts compared to AT2 H (p < 0.01). Conclusion: AT1 receptor knockout changed bone density, the quality and number of bone trabeculae, decreased the number of osteoblast cells, and increased osteonectin in fibroblasts. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Molecular Pathology, Diagnostics, and Therapeutics; https://dx.doi.org/10.3390/ijms22105217Test |
| DOI: |
10.3390/ijms22105217 |
| الإتاحة: |
https://doi.org/10.3390/ijms22105217Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.F5529426 |
| قاعدة البيانات: |
BASE |
