دورية أكاديمية
Investigating nucleo-cytoplasmic shuttling of the human DEAD-box helicase DDX3
| العنوان: | Investigating nucleo-cytoplasmic shuttling of the human DEAD-box helicase DDX3 |
|---|---|
| المؤلفون: | Brennan, Ruth, Haap-Hoff, Antje, Gua, Lili, Gautier, Virginie, Long, Aideen, Schroeder, Martina |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2018 |
| المجموعة: | Maynooth University ePrints and eTheses Archive (National University of Ireland) |
| الوصف: | The human DEAD-box helicase DDX3 is a multi-functional protein involved in the regulation of gene expression and additional non-conventional roles as signalling adaptor molecule that are independent of its enzymatic RNA remodeling activity. It is a nucleo-cytoplasmic shuttling protein and it has previously been suggested that dysregulation of its subcellular localization could contribute to tumourigenesis. Indeed, both tumour suppressor and oncogenic functions have been attributed to DDX3. In this study, we investigated the regulation of DDX3's nucleocytoplasmic shuttling. We confirmed that an N-terminal conserved Nuclear Export Signal (NES) is required for export of human DDX3 from the nucleus, and identified three regions within DDX3 that can independently facilitate its nuclear import. We also aimed to identify conditions that alter DDX3's subcellular localisation. Viral infection, cytokine treatment and DNA damage only induced minor changes in DDX3's subcellular distribution as determined by High Content Analysis. However, DDX3's nuclear localization increased in early mitotic cells (during prophase) concomitant with an increase in DDX3 expression levels. Our results are likely to have implications for the proposed use of (nuclear) DDX3 as a prognostic biomarker in cancer. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | https://mural.maynoothuniversity.ie/13711/1/MS-Investigating-2018.pdfTest; Brennan, Ruth and Haap-Hoff, Antje and Gua, Lili and Gautier, Virginie and Long, Aideen and Schroeder, Martina (2018) Investigating nucleo-cytoplasmic shuttling of the human DEAD-box helicase DDX3. European Journal of Cell Biology, 97 (7). pp. 501-511. ISSN 0171-9335 |
| الإتاحة: | https://mural.maynoothuniversity.ie/13711Test/ https://mural.maynoothuniversity.ie/13711/1/MS-Investigating-2018.pdfTest |
| رقم الانضمام: | edsbas.977BC0B2 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |