رسالة جامعية
Potencial de aplicação de marcadores RAPD e de enraizamento de estacas e miniestacas de clones de erva-mate ; Application potential of RAPD markers and rooting of cuttings and minicuttings of mate clones
| العنوان: | Potencial de aplicação de marcadores RAPD e de enraizamento de estacas e miniestacas de clones de erva-mate ; Application potential of RAPD markers and rooting of cuttings and minicuttings of mate clones |
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| المؤلفون: | Silveira, Ronilda Terezinha |
| المساهمون: | Bisognin, Dilson Antônio, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4791129Y6Test, Fleig, Frederico Dimas, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797343E4Test, Boligon, Alexandra Augusti, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4221434U8Test |
| بيانات النشر: | Universidade Federal de Santa Maria BR Recursos Florestais e Engenharia Florestal UFSM Programa de Pós-Graduação em Engenharia Florestal |
| سنة النشر: | 2012 |
| المجموعة: | Manancial - Repositório Digital da UFSM (Universidade Federal de Santa Maria) |
| مصطلحات موضوعية: | Seleção, Produção de mudas, Estaquia, Miniestaquia, Ácido indol butírico, Marcadores moleculares, Selection, Plant production, Cutting, Minicutting, Indol butyric acid, Molecular markers, CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
| الوصف: | The rescue of selected adult plants and the renewing of explants constitute the greatest challenge to enable the clonal propagation of mate (Ilex paraguariensis St. Hil.). The objectives of this study were to identify and separate clones of the clonal garden by RAPD markers and to evaluate the effect of different doses of indol butyric acid (IBA) on rooting of cuttings and minicuttings of different clones of mate. Three experiments were carried out. The first, young leaves of ten clones had the DNA extracted for testing RAPD markers. The second, new shoots of stump of four clones were used to set up one bud cuttings with one half leaf. The third experiment, rooted cuttings of 20 year-old-trees were used to set up a clonal minigarden to collect minicuttings of one bud and one half leaf. All cuttings and minicuttings were treated with different doses of IBA. The RAPD technique is successful for clone separation and identification, being the primers OPP-03, OPP-06, OPP-15 and OPP-16 the most polymorphic. Single bud cuttings of new sprouts from stumps of some mate clones are capable of rooting, without the need of indole butyric acid. The clones FO10 and FO52 have different capability for rooting. Minicuttings of the clone FO10 root without indol butyric acid. ; O resgate de plantas adultas selecionadas e o rejuvenescimento dos propágulos se constituem no maior desafio para viabilizar a clonagem massal da erva-mate (Ilex paraguariensis St. Hil.). Os objetivos deste trabalho foram identificar e separar clones que compõem o jardim clonal por meio de marcadores RAPD e avaliar o efeito de diferentes doses de ácido indol butírico (AIB) no enraizamento de estacas e miniestacas de diferentes clones de erva-mate. Para tanto foram realizados três experimentos. No primeiro, folhas jovens de dez clones tiveram o DNA extraído para testar marcadores RAPD. No segundo, brotações de cepas de quatro clones foram utilizadas para preparar estacas de gema única, com folha cortada pela metade. No terceiro experimento, estacas enraizadas ... |
| نوع الوثيقة: | thesis |
| وصف الملف: | application/pdf |
| اللغة: | Portuguese |
| العلاقة: | SILVEIRA, Ronilda Terezinha. APPLICATION POTENTIAL OF RAPD MARKERS AND ROOTING OF CUTTINGS AND MINICUTTINGS OF MATE CLONES. 2012. 51 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2012.; http://repositorio.ufsm.br/handle/1/8713Test |
| الإتاحة: | http://repositorio.ufsm.br/handle/1/8713Test |
| حقوق: | Acesso Aberto |
| رقم الانضمام: | edsbas.BDF193B7 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |