دورية أكاديمية
Lipoprotein modifications by gingipains of Porphyromonas gingivalis
| العنوان: | Lipoprotein modifications by gingipains of Porphyromonas gingivalis |
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| المؤلفون: | Lönn, Johanna, Ljunggren, Stefan, Klarström-Engström, Kristin, Demirel, Isak, Bengtsson, Torbjörn, Karlsson, Helen |
| بيانات النشر: | Malmö universitet, Odontologiska fakulteten (OD) |
| سنة النشر: | 2018 |
| المجموعة: | Malmö University Electronic Publishing (MUEP) |
| مصطلحات موضوعية: | gingipains, lipoproteins, MALDI-TOF mass spectrometry, Porphyromonas gingivalis, twodimensional gel electrophoresis, Medical and Health Sciences, Medicin och hälsovetenskap |
| الوصف: | Background and objective: Several studies have shown an association between periodontitis and cardiovascular disease (CVD). Atherosclerosis is the major cause of CVD and a key event in the development of atherosclerosis is accumulation of lipoproteins within the arterial wall. Bacteria are the primary etiologic agents in periodontitis and Porphyromonas gingivalis (P. gingivalis) is the major pathogen in the disease. Several studies support the role of modified LDL in atherogenesis, however, the pathogenic stimuli that induce the changes and the mechanisms by which this occur are unknown. This study aims at identifying alterations in plasma lipoproteins induced by the periodontopathic bacteria P. gingivalis in vitro.Material and Methods: Plasma lipoproteins were isolated from whole blood treated with wild type and gingipain-mutants (lacking either the Rgp- or Kgp gingipains) of P. gingivalis by density/gradient-ultracentrifugation and studied using two-dimensional gel electrophoresis followed by matrix-assisted laser desorption ionization mass spectrometry. P. gingivalis-induced lipid peroxidation and antioxidant levels were measured by thiobarbituric acid reactive substances (TBARs) and antioxidant assay kits, respectively, and lumiaggregometry was used for reactive oxygen species (ROS) and aggregation.Results: P. gingivalis exerted substantial proteolytic effects on the lipoproteins. The Rgp gingipains were responsible for producing two apoE, as well as two apoB-100, fragments in LDL, and the Kgp gingipain produced an un-identified fragment in HDL. P. gingivalis and its different gingipain variants induced ROS, and consumed antioxidants. Both the Rgp and Kgp gingipains were involved in inducing lipid peroxidation.Conclusions: P. gingivalis has the potential to change the expression of lipoproteins in blood which may represent a crucial link between periodontitis and cardiovascular disease. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | Journal of Periodontal Research, 0022-3484, 2018, 53:3, s. 403-413; http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-6045Test; PMID 29341140; ISI:000432018000014; Scopus 2-s2.0-85040740217; Local 24763 |
| DOI: | 10.1111/jre.12527 |
| الإتاحة: | https://doi.org/10.1111/jre.12527Test http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-6045Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.6E57F581 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/jre.12527 |
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