دورية أكاديمية
Characterization of Human Cardiac Progenitor Cell Secretome
| العنوان: | Characterization of Human Cardiac Progenitor Cell Secretome |
|---|---|
| المؤلفون: | Moore, Michayla A, Conley, Barbara, Tweedie, Eric, Ryzhov, Sergey, Sawyer, Doug, Vary, Calvin |
| المصدر: | Maine Medical Center |
| بيانات النشر: | MaineHealth Knowledge Connection |
| سنة النشر: | 2022 |
| المجموعة: | MaineHealth Knowledge Connection |
| مصطلحات موضوعية: | Wnt Signaling Pathway, Endothelial Cells, Ventricular Remodeling, cytidylyl-(3'-5')-cytidine, Cause of Death, Chromatography, Ligands, Proteomics, Secretome, Tandem Mass Spectrometry, Myocardium, Stem Cells, RNA, Myocardial Infarction, Protein Sorting Signals, Heart Diseases, Infarction, Clone Cells, Enzyme-Linked Immunosorbent Assay, Polymerase Chain Reaction, Cardiology, Medical Cell Biology |
| الوصف: | Heart Disease (HD) remains the greatest cause of death worldwide. Due to the heart's poor capacity to regenerate after damage, there is a critical need for treatments that can restore cardiac cell integrity and function in HD patients. Preclinical and clinical evidence has highlighted the potential role of Cardiac Progenitor Cells (CPCs) in regulation of cardiac repair after injury, with an emerging role of CPC secreted proteins in this process. We have been studying CPCs isolated from human left ventricular myocardium characterized by their high proliferative capacity in vitro. These cells (human highly proliferative clones, hHiPC) are characterized by high expression of CD105/Endoglin and show varying potential for differentiating into endothelial cells in vitro. When injected into the mouse myocardium after surgically induced myocardial infarction (MI), individual hHiPC clones show variable effects on heart remodeling. For instance, when clone 11 was injected into the infarct zone post-MI we saw a trend towards improvement in the fractional shortening (%) compared to clone 22 and saline injected controls. We hypothesized that hHiPCs associated with improvement in cardiac remodeling after MI will have a secretome characterized by higher expression of pro-angiogenic and pro-proliferative proteins, and these are regulated by the BMP/CD105 pathway. An unbiased and global proteomics approach (LC-MS/MS) was used in our lab to identify proteins secreted from hHiPCs pre-treated with the CD105 ligand BMP9 (5 ng/mL) compared to non-treated controls. Our secretome data identified ~700 proteins in which 57% were characterized from UniProt as having a signal peptide sequence. Several pro-angiogenic secreted proteins were identified in clone 11 including CCN2, CXCL6, and TGFB2. We found a >2.5-fold significant increase in the secretion of Sclerostin (SOST) in BMP9 treated hHiPC compared to non-treated controls. SOST is a known BMP-target protein and WNT inhibitor. Previous evidence has highlighted the role of ... |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| العلاقة: | https://knowledgeconnection.mainehealth.org/mmc/2340Test; https://pubmed.ncbi.nlm.nih.gov/35554307Test/ |
| الإتاحة: | https://knowledgeconnection.mainehealth.org/mmc/2340Test https://pubmed.ncbi.nlm.nih.gov/35554307Test/ |
| رقم الانضمام: | edsbas.1A708D7F |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |