دورية أكاديمية
IL-1/inhibitory kappa B kinase epsilon-induced glycolysis augment epithelial effector function and promote allergic airways disease
| العنوان: | IL-1/inhibitory kappa B kinase epsilon-induced glycolysis augment epithelial effector function and promote allergic airways disease |
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| المؤلفون: | Qian, Xi, Aboushousha, Reem, van de Wetering, Cheryl, Chia, Shi B., Amiel, Eyal, Schneider, Robert W., van der Velden, Jos L. J., Lahue, Karolyn G., Hoagland, Daisy A., Casey, Dylan T., Daphtary, Nirav, Ather, Jennifer L., Randall, Matthew J., Aliyeva, Minara, Black, Kendall E., Chapman, David G., Lundblad, Lennart K. A., McMillan, David H., Dixon, Anne E., Anathy, Vikas, Irvin, Charles G., Poynter, Matthew E., Wouters, Emiel F. M., Vacek, Pamela M., Henket, Monique, Schleich, Florence, Louis, Renaud, van der Vliet, Albert, Janssen-Heininger, Yvonne M. W. |
| المصدر: | Qian , X , Aboushousha , R , van de Wetering , C , Chia , S B , Amiel , E , Schneider , R W , van der Velden , J L J , Lahue , K G , Hoagland , D A , Casey , D T , Daphtary , N , Ather , J L , Randall , M J , Aliyeva , M , Black , K E , Chapman , D G , Lundblad , L K A , McMillan , D H , Dixon , A E , Anathy , V , .... |
| سنة النشر: | 2018 |
| المجموعة: | Maastricht University Research Publications |
| مصطلحات موضوعية: | Asthma, house dust mite, glycolysis, IL-1, inhibitor of kappa B kinase epsilon, lactate, lactate dehydrogenase A, DENDRITIC CELL ACTIVATION, HOUSE-DUST MITE, NLRP3 INFLAMMASOME, SEVERE ASTHMA, MURINE MODEL, LUNG-CANCER, INTERLEUKIN-1, HYPERRESPONSIVENESS, MICE, RESPONSES, METABOLISM, CELL ACTIVATION, INFLAMMATION, ACTIVATION PROMOTES |
| الوصف: | Background: Emerging studies suggest that enhanced glycolysis accompanies inflammatory responses. Virtually nothing is known about the relevance of glycolysis in patients with allergic asthma. Objectives: We sought to determine whether glycolysis is altered in patients with allergic asthma and to address its importance in the pathogenesis of allergic asthma. Methods: We examined alterations in glycolysis in sputum samples from asthmatic patients and primary human nasal cells and used murine models of allergic asthma, as well as primary mouse tracheal epithelial cells, to evaluate the relevance of glycolysis. Results: In a murine model of allergic asthma, glycolysis was induced in the lungs in an IL-1-dependent manner. Furthermore, administration of IL-1 beta into the airways stimulated lactate production and expression of glycolytic enzymes, with notable expression of lactate dehydrogenase A occurring in the airway epithelium. Indeed, exposure of mouse tracheal epithelial cells to IL-1 beta or IL-1 alpha resulted in increased glycolytic flux, glucose use, expression of glycolysis genes, and lactate production. Enhanced glycolysis was required for IL-1 beta- or IL-1 alpha-mediated proinflammatory responses and the stimulatory effects of IL-1 beta on house dust mite (HDM)-induced release of thymic stromal lymphopoietin and GM-CSF from tracheal epithelial cells. Inhibitor of kappa B kinase epsilon was downstream of HDM or IL-1 beta and required for HDM-induced glycolysis and pathogenesis of allergic airways disease. Small interfering RNA ablation of lactate dehydrogenase A attenuated HDM-induced increases in lactate levels and attenuated HDM-induced disease. Primary nasal epithelial cells from asthmatic patients intrinsically produced more lactate compared with cells from healthy subjects. Lactate content was significantly higher in sputum supernatants from asthmatic patients, notably those with greater than 61% neutrophils. A positive correlation was observed between sputum lactate and IL-1 beta levels, and ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/j.jaci.2017.08.043 |
| الإتاحة: | https://doi.org/10.1016/j.jaci.2017.08.043Test https://cris.maastrichtuniversity.nl/en/publications/6be1e3fe-4aba-4a60-a183-233a452f95bdTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.4B8B787A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jaci.2017.08.043 |
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