دورية أكاديمية
A draft Diabrotica virgifera virgifera genome : insights into control and host plant adaption by a major maize pest insect
| العنوان: | A draft Diabrotica virgifera virgifera genome : insights into control and host plant adaption by a major maize pest insect |
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| المؤلفون: | Coates, Brad S., Walden, Kimberly K.O., Lata, Dimpal, Vellichirammal, Neetha Nanoth, Mitchell, Robert F., Andersson, Martin N., McKay, Rachel, Lorenzen, Marcé D., Grubbs, Nathaniel, Wang, Yu Hui, Han, Jinlong, Xuan, Jing Li, Willadsen, Peter, Wang, Huichun, French, B. Wade, Bansal, Raman, Sedky, Sammy, Souza, Dariane, Bunn, Dakota, Meinke, Lance J., Miller, Nicholas J., Siegfried, Blair D., Sappington, Thomas W., Robertson, Hugh M. |
| المصدر: | BMC Genomics; 24(1), no 19 (2023) ; ISSN: 1471-2164 |
| بيانات النشر: | BioMed Central (BMC) |
| سنة النشر: | 2023 |
| المجموعة: | Lund University Publications (LUP) |
| مصطلحات موضوعية: | Bioinformatics and Systems Biology, Agricultural Science, Differential expression, Genome assembly, Host plant specialization |
| الوصف: | Background: Adaptations by arthropod pests to host plant defenses of crops determine their impacts on agricultural production. The larval host range of western corn rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae), is restricted to maize and a few grasses. Resistance of D. v. virgifera to crop rotation practices and multiple insecticides contributes to its status as the most damaging pest of cultivated maize in North America and Europe. The extent to which adaptations by this pest contributes to host plant specialization remains unknown. Results: A 2.42 Gb draft D. v. virgifera genome, Dvir_v2.0, was assembled from short shotgun reads and scaffolded using long-insert mate-pair, transcriptome and linked read data. K-mer analysis predicted a repeat content of ≥ 61.5%. Ortholog assignments for Dvir_2.0 RefSeq models predict a greater number of species-specific gene duplications, including expansions in ATP binding cassette transporter and chemosensory gene families, than in other Coleoptera. A majority of annotated D. v. virgifera cytochrome P450s belong to CYP4, 6, and 9 clades. A total of 5,404 transcripts were differentially-expressed between D. v. virgifera larvae fed maize roots compared to alternative host (Miscanthus), a marginal host (Panicum virgatum), a poor host (Sorghum bicolor) and starvation treatments; Among differentially-expressed transcripts, 1,908 were shared across treatments and the least number were between Miscanthus compared to maize. Differentially-expressed transcripts were enriched for putative spliceosome, proteosome, and intracellular transport functions. General stress pathway functions were unique and enriched among up-regulated transcripts in marginal host, poor host, and starvation responses compared to responses on primary (maize) and alternate hosts. Conclusions: Manual annotation of D. v. virgifera Dvir_2.0 RefSeq models predicted expansion of paralogs with gene families putatively involved in insecticide resistance and chemosensory perception. Our study also ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://lup.lub.lu.se/record/da811c78-b06d-4b27-a7d0-bcdd0599cf6fTest; http://dx.doi.org/10.1186/s12864-022-08990-yTest; pmid:36639634; scopus:85146293274 |
| DOI: | 10.1186/s12864-022-08990-y |
| الإتاحة: | https://doi.org/10.1186/s12864-022-08990-yTest https://lup.lub.lu.se/record/da811c78-b06d-4b27-a7d0-bcdd0599cf6fTest |
| رقم الانضمام: | edsbas.613187C7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12864-022-08990-y |
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