دورية أكاديمية
Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR
| العنوان: | Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR |
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| المؤلفون: | Krokowski, Dawid, Jobava, Raul, Szkop, Krzysztof J, Chen, Chien-Wen, Fu, Xu, Venus, Sarah, Guan, Bo-Jhih, Wu, Jing |
| المصدر: | Faculty Publications |
| بيانات النشر: | LSU Digital Commons |
| سنة النشر: | 2022 |
| المجموعة: | LSU Digital Commons (Louisiana State University) |
| مصطلحات موضوعية: | CP: Molecular biology, amino acids, cytoplasmic condensates, hypertonic stress, mTOR signaling, osmolytes, osmotolerance, translation |
| الوصف: | The integrated stress response (ISR) plays a pivotal role in adaptation of translation machinery to cellular stress. Here, we demonstrate an ISR-independent osmoadaptation mechanism involving reprogramming of translation via coordinated but independent actions of mTOR and plasma membrane amino acid transporter SNAT2. This biphasic response entails reduced global protein synthesis and mTOR signaling followed by translation of SNAT2. Induction of SNAT2 leads to accumulation of amino acids and reactivation of mTOR and global protein synthesis, paralleled by partial reversal of the early-phase, stress-induced translatome. We propose SNAT2 functions as a molecular switch between inhibition of protein synthesis and establishment of an osmoadaptive translation program involving the formation of cytoplasmic condensates of SNAT2-regulated RNA-binding proteins DDX3X and FUS. In summary, we define key roles of SNAT2 in osmotolerance. |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| العلاقة: | https://digitalcommons.lsu.edu/biosci_pubs/4668Test |
| DOI: | 10.1016/j.celrep.2022.111092 |
| الإتاحة: | https://doi.org/10.1016/j.celrep.2022.111092Test https://digitalcommons.lsu.edu/biosci_pubs/4668Test |
| رقم الانضمام: | edsbas.F4AB3EAE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.celrep.2022.111092 |
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