دورية أكاديمية
Localization and characterization of phenamil-sensitive Na + influx in isolated rainbow trout gill epithelial cells
| العنوان: | Localization and characterization of phenamil-sensitive Na + influx in isolated rainbow trout gill epithelial cells |
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| المؤلفون: | Reid, Scott D., Hawkings, G. S., Galvez, F., Goss, G. G. |
| المصدر: | Faculty Publications |
| بيانات النشر: | LSU Digital Commons |
| سنة النشر: | 2003 |
| المجموعة: | LSU Digital Commons (Louisiana State University) |
| مصطلحات موضوعية: | CC, Chloride cells, Density gradient, Fish, Mitochondria-rich cells, MR, Na influx +, Pavement cells, Peanut lectin agglutinin, PNA, PVC, Transport |
| الوصف: | Percoll density-gradient separation, combined with peanut lectin agglutinin (PNA) binding and magnetic bead separation, was used to separate dispersed fish gill cells into sub-populations. Functional characterization of each of the sub-populations was performed to determine which displayed acid-activated phenamil- and bafilomycin-sensitive Na+ uptake. Analysis of the mechanism(s) of 22Na+ influx was performed in control and acid-activated (addition of 10 mmoll-1 proprionic acid) cells using a variety of Na+ transport inhibitors (ouabain, phenamil, HOE-694 and bumetanide) and a V-type ATPase inhibitor (bafilomycin). We found that cells migrating to a 1.03-1.05 g ml-1 Percoll interface [pavement cells (PVCs)] possessed the lowest rates of Na+ uptake and that influx was unchanged during either bafilomycin (10 nmoll-1) treatment or internal acidification with addition of proprionic acid (10 mmoll-1). Mitochondria-rich (MR) cells that migrated to the 1.05-1.09 g ml-1 interface of the Percoll gradient demonstrated acidification-activated bafilomycin and phenamil-sensitive Na+ influx. Further separation of the MR fraction into PNA+ and PNA- fractions using magnetic separation demonstrated that only the PNA- cells (α-MR cells) demonstrated phenamiland bafilomycin-sensitive acid-activated 22Na+ uptake. We confirm the coupling of a V-type H+-ATPase with phenamil-sensitive Na+ uptake activity and conclude that high-density α-MR cells function in branchial Na+ uptake in freshwater fish. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://digitalcommons.lsu.edu/biosci_pubs/1259Test; https://digitalcommons.lsu.edu/context/biosci_pubs/article/2258/viewcontent/1259.pdfTest |
| DOI: | 10.1242/jeb.00109 |
| الإتاحة: | https://doi.org/10.1242/jeb.00109Test https://digitalcommons.lsu.edu/biosci_pubs/1259Test https://digitalcommons.lsu.edu/context/biosci_pubs/article/2258/viewcontent/1259.pdfTest |
| رقم الانضمام: | edsbas.55F58062 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1242/jeb.00109 |
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