دورية أكاديمية
Development of DNA Markers From Physically Mapped Loci in Aegilops comosa and Aegilops umbellulata Using Single-Gene FISH and Chromosome Sequences
العنوان: | Development of DNA Markers From Physically Mapped Loci in Aegilops comosa and Aegilops umbellulata Using Single-Gene FISH and Chromosome Sequences |
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المؤلفون: | Said, Mahmoud, Holusova, Katerina, Farkas, Andras, Ivanizs, Laszlo, Gaal, Eszter, Capal, Petr, Abrouk, Michael, Martis-Thiele, Mihaela-Maria, Kalapos, Balazs, Bartos, Jan, Friebe, Bernd, Dolezel, Jaroslav, Molnar, Istvan |
بيانات النشر: | Linköpings universitet, Medicinska fakulteten Linköpings universitet, Avdelningen för cellbiologi Czech Acad Sci, Czech Republic; Agr Res Ctr, Egypt Czech Acad Sci, Czech Republic ELKH Ctr Agr Res, Hungary King Abdullah Univ Sci & Technol, Saudi Arabia Kansas State Univ, KS 66506 USA Czech Acad Sci, Czech Republic; ELKH Ctr Agr Res, Hungary Frontiers Media SA |
سنة النشر: | 2021 |
المجموعة: | Linköping University Electronic Press (LiU E-Press) |
مصطلحات موضوعية: | goat grasses, Aegilops comosa, Aegilops umbellulata, single-gene FISH, chromosome flow sorting and sequencing, chromosome rearrangements, homoeologous relationships, molecular markers, Biochemistry and Molecular Biology, Biokemi och molekylärbiologi |
الوصف: | Breeding of agricultural crops adapted to climate change and resistant to diseases and pests is hindered by a limited gene pool because of domestication and thousands of years of human selection. One way to increase genetic variation is chromosome-mediated gene transfer from wild relatives by cross hybridization. In the case of wheat (Triticum aestivum), the species of genus Aegilops are a particularly attractive source of new genes and alleles. However, during the evolution of the Aegilops and Triticum genera, diversification of the D-genome lineage resulted in the formation of diploid C, M, and U genomes of Aegilops. The extent of structural genome alterations, which accompanied their evolution and speciation, and the shortage of molecular tools to detect Aegilops chromatin hamper gene transfer into wheat. To investigate the chromosome structure and help develop molecular markers with a known physical position that could improve the efficiency of the selection of desired introgressions, we developed single-gene fluorescence in situ hybridization (FISH) maps for M- and U-genome progenitors, Aegilops comosa and Aegilops umbellulata, respectively. Forty-three ortholog genes were located on 47 loci in Ae. comosa and on 52 loci in Ae. umbellulata using wheat cDNA probes. The results obtained showed that M-genome chromosomes preserved collinearity with those of wheat, excluding 2 and 6M containing an intrachromosomal rearrangement and paracentric inversion of 6ML, respectively. While Ae. umbellulata chromosomes 1, 3, and 5U maintained collinearity with wheat, structural reorganizations in 2, 4, 6, and 7U suggested a similarity with the C genome of Aegilops markgrafii. To develop molecular markers with exact physical positions on chromosomes of Aegilops, the single-gene FISH data were validated in silico using DNA sequence assemblies from flow-sorted M- and U-genome chromosomes. The sequence similarity search of cDNA sequences confirmed 44 out of the 47 single-gene loci in Ae. comosa and 40 of the 52 map positions in ... |
نوع الوثيقة: | article in journal/newspaper |
وصف الملف: | application/pdf |
اللغة: | English |
العلاقة: | Frontiers in Plant Science, 2021, 12; http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-180119Test; PMID 34211490; ISI:000667808800001 |
DOI: | 10.3389/fpls.2021.689031 |
الإتاحة: | https://doi.org/10.3389/fpls.2021.689031Test http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-180119Test |
حقوق: | info:eu-repo/semantics/openAccess |
رقم الانضمام: | edsbas.DF6E6C8E |
قاعدة البيانات: | BASE |
DOI: | 10.3389/fpls.2021.689031 |
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