رسالة جامعية
Seminal Influence on the Oviduct : Mating and/or semen components induce gene expression changes in the pre-ovulatory functional sperm reservoir in poultry and pigs
| العنوان: | Seminal Influence on the Oviduct : Mating and/or semen components induce gene expression changes in the pre-ovulatory functional sperm reservoir in poultry and pigs |
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| المؤلفون: | Atikuzzaman, Mohammad |
| بيانات النشر: | Linköpings universitet, Avdelningen för kliniska vetenskaper Linköpings universitet, Medicinska fakulteten Linköping |
| سنة النشر: | 2016 |
| المجموعة: | Linköping University Electronic Press (LiU E-Press) |
| مصطلحات موضوعية: | Seminal plasma, proteome/peptidome, oviduct, gene expression, chicken, pig, Genetics, Genetik, Clinical Science, Klinisk vetenskap, Immunology in the medical area, Immunologi inom det medicinska området |
| الوصف: | Internal fertilization occurs in birds and eutherian mammals. Foetal development, however, is either extra- respectively intra-corpore (egg vs uterus). In these animal classes, the female genital tract stores ejaculated spermatozoa into a restricted oviductal segment; the functional pre-ovulatory sperm reservoir, where they survive until ovulation/s occur. Paradoxically, this immunologically foreign sperm suspension in seminal fluid/plasma, often microbiologically contaminated, ought to be promptly eliminated by the female local immune defence which, instead, tolerates its presence. The female immune tolerance is presumably signalled via a biochemical interplay of spermatozoa, as well as the peptides and proteins of the extracellular seminal fluid, with female epithelial and immune cells. Such interplay can result in gene expression shifts in the sperm reservoir in relation to variations in fertility. To further aid our understanding of the underlying mechanisms, this thesis studied the proteome of the seminal fluid (using 2D SDS-PAGE and mass spectrometry) including cytokine content (using Luminex and/or ELISA) of healthy, sexually mature and fertile boars and cocks. As well, gene expression changes (using cDNA microarray) in the oviductal sperm reservoirs of sexually-mature females, mated or artificially infused with homologous sperm-free seminal fluid/plasma were studied. Pigs were of commercial, fertility-selected modern breeds (Landrace), while chicken belonged to the ancestor Red Junglefowl (RJF, low egg laying-capacity), a selected egg-layer White Leghorn (WL) and of their Advanced Intercross Line (AIL). Ejaculates were manually collected as single sample in cocks or as the sperm-rich fraction [SRF] and the post- SRF fraction in boars to harvest seminal fluid/plasma for proteome/cytokine and infusion-studies. Oviducts were retrieved for gene-expression analyses via microarray immediately post-mortem (chicken) or at surgery (pig), 24 h after mating or genital infusion. In pigs, the protein-rich seminal ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-91-7685-704-5 91-7685-704-2 |
| العلاقة: | Linköping University Medical Dissertations, 0345-0082; 1533; http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-132621Test; urn:isbn:9789176857045 |
| DOI: | 10.3384/diss.diva-132621 |
| الإتاحة: | https://doi.org/10.3384/diss.diva-132621Test http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-132621Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.220E5860 |
| قاعدة البيانات: | BASE |
| ردمك: | 9789176857045 9176857042 |
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| DOI: | 10.3384/diss.diva-132621 |