دورية أكاديمية
Modelling of immunosensor response: the evaluation of binding kinetics between an immobilized receptor and structurally-different genetically engineered ligands
| العنوان: | Modelling of immunosensor response: the evaluation of binding kinetics between an immobilized receptor and structurally-different genetically engineered ligands |
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| المؤلفون: | Balevičius, Zigmas, Talbot, Julian, Tamošaitis, Linas, Plikusienė, Ieva, Stirkė, Arūnas, Mickienė, Gitana, Balevičius, Saulius, Paulauskas, Andrius, Ramanavičius, Arūnas |
| المصدر: | Sensors and actuators B: Chemical, Lausanne : Elsevier Science, 2019, vol. 297, art. no. 126770, p. [1-8] ; ISSN 0925-4005 |
| سنة النشر: | 2019 |
| المجموعة: | LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) / LAEI VB (Lietuvos agrarinės ekonomikos institutasvirtualią biblioteką) |
| مصطلحات موضوعية: | immunosensor, receptor-ligand interaction, spectroscopic ellipsometry, total internal reflection ellipsometry, granulocyte colony stimulating factor (GCSF), langmuir kinetics model, three-stage kinetics model |
| الوصف: | In this research we have evaluated the binding kinetics between an immobilized receptor and several genetically engineered ligands, differing by molecular mass or by the number of binding sites available for the binding to the receptor. Genetically engineered protein (GCSF-Receptor), which contains some antibody parts (Fc domain) and at some extent is similar to antibody because also has two binding sites that selectively bind another protein – glycoprotein granulocyte colony stimulating factor (GCSF), which was immobilized on a thin gold layer in order to design an immunosensor sensitive to GCSF. Three structurally different GCSF-based proteins were genetically-engineered and evaluated as ligands, which selectively bind to immobilized GCSF-Receptor: (i) GCSF monomer (mGCSF), (ii) GCSF-homodimer consisting of two via polypeptide Lα-based linker ‘fused’ GCSF molecules ((GCSF)2Lα) and (iii) GCSF-heterodimer (SCF-Lα-GCSF), which is based on a native GCSF molecule ‘fused’ via Lα-based linker with another protein – a soluble part of stem cell factor (SCF). SCF, unlike GCSF, does not contain any site suitable for GCSF-Receptor binding. The ligands differ by: (i) molecular mass – (GCSF)2Lα and SCF-Lα-GCSF F are two times heavier than mGCS, (ii) number of binding sites – mGCSF and SCF-Lα-GCSF have one binding site, while (GCSF)2Lα has two. The binding kinetics of mGCSF, (GCSF)2Lα, and SCF-Lα-GCSF with immobilized GCSF-Receptor was investigated using total internal reflection ellipsometry. The interaction kinetics of the mGCSF and SCF-Lα-GCSF are both well described using a standard Langmuir kinetics model. However, receptor-ligand association and dissociation rates in the case of SCF-Lα-GCSF ligand are about 10 times lower than that of mGCSF. The association rate of (GCSF)2Lα is about half of that of the mGCSF, which can be explained by the smaller diffusion coefficient of the larger molecule. Moreover, unlike SCF-Lα-GCSF, the (GCSF)2Lα adsorption kinetics cannot be adequately described by the standard Langmuir kinetics ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | http://vu.lvb.lt/VU:ELABAPDB39906585&prefLang=en_USTest |
| الإتاحة: | https://doi.org/10.1016/j.snb.2019.126770Test http://vu.lvb.lt/VU:ELABAPDB39906585&prefLang=en_USTest |
| رقم الانضمام: | edsbas.F0586F2D |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |