رسالة جامعية
Hemolizinių anemijų laboratorinis vertinimas ; Laboratory evaluation of hemolytic anemia
| العنوان: | Hemolizinių anemijų laboratorinis vertinimas ; Laboratory evaluation of hemolytic anemia |
|---|---|
| المؤلفون: | Vertelytė, Dalia |
| المساهمون: | Urbonienė, Daiva |
| بيانات النشر: | Institutional Repository of Lithuanian University of Health Sciences |
| سنة النشر: | 2018 |
| المجموعة: | LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) / LAEI VB (Lietuvos agrarinės ekonomikos institutasvirtualią biblioteką) |
| مصطلحات موضوعية: | hemolytic anemia, paroxysmal nocturnal hemoglobinuria, lactate dehydrogenase activity ratio with its upper reference limit |
| الوصف: | Author of a master‘s thesis. Dalia Vertelytė The title of the master‘s thesis. Laboratory evaluation of hemolytic anemia. The aim: To evaluate changes of laboratory tests during hemolytic anemia and relate them with of type of hemolytic anemia. Objectives: To evaluate hematology test results, lactate dehydrogenase (LDH) activity, haptoglobin (HAPTO) concentration and direct antiglobulin test (DAT) in patients with hemolytic status; To compare groups of patients according to levels of LDH activity to LDH upper reference limit ratio LDH/URL; To evuluate the changes of laboratory parameters in patients with hemolytic anemia according to presence of clone of paroxysmal norturnal hemoglobinuria (PNH). Patients and methods. Patients with disorders of hemopoiesis were included in the study, then patients tested for HAPTO and LDH were chosen, and additional selection was done – 80 patients with HAPTO concentration lower than 360 mg/l were left for the final analysis. 26 patients were included in flow cytometry analysis. Hematology tests were done using automated hematologic s XE–5000 (Sysmex, Japan). The biochemistry tests were run using clinical system – SYNCHRON UniCel® DxC 800 (Beckman Coulter, USA) and DAT were tested by column hemagglutination technique (Biorad, USA). The immunophenotyping of RBC and WBC was carried out for identification of PNH clone using flow cytometre BD FacsCanto (BD Immunocytometry Systems, USA). Results. The prevalence of negative DAT results didn‘t differ between male and female patients with hemolytic anemias. RBC, HGB, HCT values were significantly lower and TBIL – significantly higher in patients with LDH/URL1.5. Spearman‘s correlative test revealed reverse significant correlation between LDH and HAPTO (R=-0,239; p=0,033). The anemia markers –RBC and HGB significantly negatively correlated with the LDH. Frequency of markers characteristic to PNH (combination of DAT negative results and increased LDH) didn‘t differ between male and female patients. Patients having PNH clone had 12-fold ... |
| نوع الوثيقة: | master thesis |
| وصف الملف: | application/pdf |
| اللغة: | Lithuanian English |
| العلاقة: | http://lsmu.oai.elaba.lt/documents/28919948.pdfTest; http://lsmu.lvb.lt/LSMU:ELABAETD28919948&prefLang=en_USTest |
| الإتاحة: | http://lsmu.oai.elaba.lt/documents/28919948.pdfTest http://lsmu.lvb.lt/LSMU:ELABAETD28919948&prefLang=en_USTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.24989734 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |