دورية أكاديمية
Structure-activity relationship study of the neuritogenic potential of the glycan of starfish ganglioside LLG-3
| العنوان: | Structure-activity relationship study of the neuritogenic potential of the glycan of starfish ganglioside LLG-3 |
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| المؤلفون: | Yamagishi, Megumi, Hosoda-Yabe, Ritsuko, Tamai, Hideki, Konishi, Miku, Imamura, Akihiro, Ishida, Hideharu, Yabe, Tomio, Ando, Hiromune, Kiso, Makoto |
| المساهمون: | 木曽, 真 |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2015 |
| المجموعة: | Kyoto University Research Information Repository (KURENAI) / 京都大学学術情報リポジトリ |
| مصطلحات موضوعية: | ganglioside, starfish, neurite outgrowth, PC12 cell, SH‐SY5Y cell, structure‐activity relationship, MAPK/ERK signaling |
| الوصف: | LLG-3 is a ganglioside isolated from the starfish Linchia laevigata. To clarify the structure-activity relationship of the glycan of LLG-3 toward rat pheochromocytoma PC12 cells in the presence of nerve growth factor, a series of mono- to tetrasaccharide glycan derivatives were chemically synthesized and evaluated in vitro. The methyl group at C8 of the terminal sialic acid residue was crucial for neuritogenic activity, and the terminal trisaccharide moiety was the minimum active motif. Furthermore, the trisaccharide also stimulated neuritogenesis in human neuroblastoma SH-SY5Y cells via mitogen-activated protein kinase (MAPK) signaling. Phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was rapidly induced by adding 1 or 10 nM of the trisaccharide. The ratio of phosphorylated ERK to ERK reached a maximum 5 min after stimulation, and then decreased gradually. However, the trisaccharide did not induce significant Akt phosphorylation. These effects were abolished by pretreatment with the MAPK inhibitor U0126, which inhibits enzymes MEK1 and MEK2. In addition, U0126 inhibited the phosphorylation of ERK 1/2 in response to the trisaccharide dose-dependently. Therefore, we concluded that the trisaccharide promotes neurite extension in SH-SY5Y cells via MAPK/ERK signaling, not Akt signaling. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1660-3397 26690179 |
| العلاقة: | http://hdl.handle.net/2433/210692Test; Marine Drugs; 13; 12; 7250; 7274 |
| الإتاحة: | http://hdl.handle.net/2433/210692Test |
| حقوق: | © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CCBY) license (http://creativecommons.org/licenses/by/4.0Test/). |
| رقم الانضمام: | edsbas.8029A0D2 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 16603397 26690179 |
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