دورية أكاديمية
Crucial role of vinexin for keratinocyte migration in vitro and epidermal wound healing in vivo.
| العنوان: | Crucial role of vinexin for keratinocyte migration in vitro and epidermal wound healing in vivo. |
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| المؤلفون: | Kioka, Noriyuki, Ito, Takuya, Yamashita, Hiroshi, Uekawa, Natsuko, Umemoto, Tsutomu, Motoyoshi, Soh, Imai, Hiroshi, Takahashi, Kenzo, Watanabe, Hideto, Yamada, Masayasu, Ueda, Kazumitsu |
| المساهمون: | 木岡, 紀幸, 90234179, 10303869, 10243073, 10151789 |
| بيانات النشر: | Elsevier Inc. |
| سنة النشر: | 2010 |
| المجموعة: | Kyoto University Research Information Repository (KURENAI) / 京都大学学術情報リポジトリ |
| مصطلحات موضوعية: | Keratinocytes, Migration, Wound healing, EGFR, Vinexin, Adaptor Proteins, Signal Transducing/antagonists & inhibitors, Signal Transducing/genetics, Signal Transducing/physiology, Animals, Base Sequence, Cell Line, Cell Movement/physiology, Cell Proliferation, Cells, Cultured, Extracellular Signal-Regulated MAP Kinases/physiology, Humans, Keratinocytes/cytology, Keratinocytes/physiology, Mice, Inbred C57BL, Knockout, Muscle Proteins/deficiency, Muscle Proteins/genetics, Muscle Proteins/physiology, RNA, Small Interfering/genetics, Receptor, Epidermal Growth Factor/physiology |
| الوصف: | In the process of tissue injury and repair, epithelial cells rapidly migrate and form epithelial sheets. Vinexin is a cytoplasmic molecule of the integrin-containing cell adhesion complex localized at focal contacts in vitro. Here, we investigated the roles of vinexin in keratinocyte migration in vitro and wound healing in vivo. Vinexin knockdown using siRNA delayed migration of both HaCaT human keratinocytes and A431 epidermoid carcinoma cells in scratch assay but did not affect cell proliferation. Induction of cell migration by scratching the confluent monolayer culture of these cells activated both EGFR and ERK, and their inhibitors AG1478 and U0126 substantially suppressed scratch-induced keratinocyte migration. Vinexin knockdown in these cells inhibited the scratch-induced activation of EGFR, but not that of ERK, suggesting that vinexin promotes cell migration via activation of EGFR. We further generated vinexin (-/-) mice and isolated their keratinocytes. They similarly showed slow migration in scratch assay. Furthermore, vinexin (-/-) mice exhibited a delay in cutaneous wound healing in both the back skin and tail without affecting the proliferation of keratinocytes. Together, these results strongly suggest a crucial role of vinexin in keratinocyte migration in vitro and cutaneous wound healing in vivo. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1090-2422 20361963 |
| العلاقة: | http://hdl.handle.net/2433/130802Test; AA00641011; Experimental cell research; 316; 10; 1728; 1738 |
| الإتاحة: | http://hdl.handle.net/2433/130802Test |
| حقوق: | © 2010 Elsevier Inc. ; This is not the published version. Please cite only the published version. ; この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
| رقم الانضمام: | edsbas.A721175F |
| قاعدة البيانات: | BASE |
| تدمد: | 10902422 20361963 |
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