التفاصيل البيبلوغرافية
| العنوان: |
Monitoring ATP dynamics in electrically active white matter tracts |
| المؤلفون: |
Trevisiol, Andrea, Saab, Aiman S, Winkler, Ulrike, Marx, Grit, Imamura, Hiromi, Möbius, Wiebke, Kusch, Kathrin, Nave, Klaus-Armin, Hirrlinger, Johannes |
| المساهمون: |
今村, 博臣, 20422545 |
| بيانات النشر: |
eLife Sciences Organisation, Ltd. |
| سنة النشر: |
2017 |
| المجموعة: |
Kyoto University Research Information Repository (KURENAI) / 京都大学学術情報リポジトリ |
| الوصف: |
In several neurodegenerative diseases and myelin disorders, the degeneration profiles of myelinated axons are compatible with underlying energy deficits. However, it is presently impossible to measure selectively axonal ATP levels in the electrically active nervous system. We combined transgenic expression of an ATP-sensor in neurons of mice with confocal FRET imaging and electrophysiological recordings of acutely isolated optic nerves. This allowed us to monitor dynamic changes and activity-dependent axonal ATP homeostasis at the cellular level and in real time. We find that changes in ATP levels correlate well with compound action potentials. However, this correlation is disrupted when metabolism of lactate is inhibited, suggesting that axonal glycolysis products are not sufficient to maintain mitochondrial energy metabolism of electrically active axons. The combined monitoring of cellular ATP and electrical activity is a novel tool to study neuronal and glial energy metabolism in normal physiology and in models of neurodegenerative disorders. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
2050-084X |
| العلاقة: |
http://hdl.handle.net/2433/226313Test; eLife; e24241 |
| الإتاحة: |
http://hdl.handle.net/2433/226313Test |
| حقوق: |
Copyright Trevisiol et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. |
| رقم الانضمام: |
edsbas.5BF7FDAB |
| قاعدة البيانات: |
BASE |
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