التفاصيل البيبلوغرافية
| العنوان: |
Production of Extrachromosomal MicroDNAs Is Linked to Mismatch Repair Pathways and Transcriptional Activity |
| المؤلفون: |
Dillon, Laura W., Kumar, Pankaj, Shibata, Yoshiyuki, Wang, Yuh Hwa, Willcox, Smaranda, Griffith, Jack D., Pommier, Yves, Takeda, Shunichi, Dutta, Anindya |
| المساهمون: |
武田, 俊一 |
| بيانات النشر: |
Elsevier |
| سنة النشر: |
2015 |
| المجموعة: |
Kyoto University Research Information Repository (KURENAI) / 京都大学学術情報リポジトリ |
| الوصف: |
MicroDNAs are <400-base extrachromosomal circles found in mammalian cells. Tens of thousands of microDNAs have been found in all tissue types, including sperm. MicroDNAs arise preferentially from areas with high gene density, GC content, and exon density from promoters with activating chromatin modifications and in sperm from the 5'-UTR of full-length LINE-1 elements, but are depleted from lamin-associated heterochromatin. Analysis of microDNAs from a set of human cancer cell lines revealed lineage-specific patterns of microDNA origins. A survey of microDNAs from chicken cells defective in various DNA repair proteins reveals that homologous recombination and non-homologous end joining repair pathways are not required for microDNA production. Deletion of the MSH3 DNA mismatch repair protein results in a significant decrease in microDNA abundance, specifically from non-CpG genomic regions. Thus, microDNAs arise as part of normal cellular physiology-either from DNA breaks associated with RNA metabolism or from replication slippage followed by mismatch repair. Through isolating and sequencing small extrachromosomal circular microDNAs across multiple species and cell types, Dillon et al. identify specific genomic features associated with the generation of microDNAs and link the DNA mismatch repair pathway to this process. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
2211-1247 |
| العلاقة: |
http://hdl.handle.net/2433/216328Test; Cell Reports; 11; 1749; 1759 |
| الإتاحة: |
http://hdl.handle.net/2433/216328Test |
| حقوق: |
© 2015 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0Test/). |
| رقم الانضمام: |
edsbas.A0A80D30 |
| قاعدة البيانات: |
BASE |
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