دورية أكاديمية
Capsaicin suppresses ciliary function, while inducing permeability in bronchial epithelial cell cultures of COPD patients
| العنوان: | Capsaicin suppresses ciliary function, while inducing permeability in bronchial epithelial cell cultures of COPD patients |
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| المساهمون: | Yıldız, Abdullah Burak, Bayram, Hasan (ORCID 0000-0002-5236-766X & YÖK ID 4890), Toy, H.I., Taşdemir, Kahraman D., İlhan, S., Dikensoy, O., Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM), School of Medicine |
| المصدر: | Frontiers in Pharmacology |
| بيانات النشر: | Frontiers International |
| سنة النشر: | 2022 |
| المجموعة: | Koç University Suna Kıraç Library’ Digital Collections |
| مصطلحات موضوعية: | Medicine, Pharmacology, Pharmacy, Airways, Ciliary beat frequency (CBF), Cytokine, Formoterol, Roflumilast, Tear gas, Transepithelial electrical resistance (TEER) |
| الوصف: | Take home message: Capsaicin modified inflammatory response and caused toxicity in bronchial epithelial cultures from patients with COPD. More importantly, capsaicin decreased ciliary beat frequency and induced epithelial permeability and these effects were partially prevented by formoterol and roflumilast. Tear gas is widely used to halt mass demonstrations. Studies have reported its adverse effects on multiple organ systems; however, its effect on individuals with chronic respiratory diseases and the underlying mechanisms of these effects are unclear. For the first time in the literature, we investigated the effects of capsaicin, the active ingredient of tear gas, on bronchial epithelial cell (BEC) cultures obtained from well-characterized groups of nonsmokers, smokers, and patients with chronic obstructive pulmonary disease (COPD). BEC cultures were incubated with 50-500 ?M capsaicin in the absence and presence of formoterol (1?M) and roflumilast (0.1 ?M) for 24 h. Ciliary beat frequency (CBF) and transepithelial electrical resistance (TEER) were assessed at T1/4, T1/2, T1, T2, T4, T6, and T24 h, whereas the release of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-8, and lactate dehydrogenase (LDH) was measured at T24 h. Capsaicin (250 µM) significantly decreased CBF of all BEC cultures from T1/4 h to T24 h (p<0.05). Formoterol significantly prevented decreases in CBF induced by capsaicin. Higher concentrations of capsaicin (250-500 ?M) significantly reduced TEER of BECs from nonsmokers (T2-T24 h), smokers (T24 h) and COPD patients (T2 and T24 h), which was partially prevented by roflumilast. Capsaicin (500 ?M) decreased release of IL-8 (p<0.0001) and GM-CSF (p<0.05) while inducing release of LDH in BECs (p<0.05), and this was more prominent in BEC from patients with COPD. In conclusion, our findings demonstrate that capsaicin can suppress ciliary activity and cytokine release from BECs, induce BEC culture permeability and cellular toxicity and that these effects can ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | |
| اللغة: | English |
| تدمد: | 1663-9812 |
| العلاقة: | Publisher version; Koç University Institutional Repository; Halil Ibrahim et al. “Capsaicin Suppresses Ciliary Function While Inducing Permeability in Bronchial Epithelial Cell Cultures of Copd Patients.”. 2022.; https://dx.doi.org/10.3389/fphar.2022.996046Test; IR04020.pdf; WoS; Scopus; PubMed; NA; http://libdigitalcollections.ku.edu.tr/cdm/ref/collection/IR/id/10900Test |
| DOI: | 10.3389/fphar.2022.996046 |
| الإتاحة: | https://doi.org/10.3389/fphar.2022.996046Test http://libdigitalcollections.ku.edu.tr/cdm/ref/collection/IR/id/10900Test |
| رقم الانضمام: | edsbas.765C0594 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 16639812 |
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| DOI: | 10.3389/fphar.2022.996046 |