دورية أكاديمية
Cetuximab-Ag2S quantum dots for fluorescence imaging and highly effective combination of ALA-based photodynamic/chemo-therapy of colorectal cancer cells
| العنوان: | Cetuximab-Ag2S quantum dots for fluorescence imaging and highly effective combination of ALA-based photodynamic/chemo-therapy of colorectal cancer cells |
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| المساهمون: | Acar, Funda Havva Yağcı (ORCID 0000-0001-5601-8814 & YÖK ID 178902), Bayır, Ali, Hashemkhani, Mahshid, Demirci, Gözde, Muti, Abdullah, Sennaroğlu, Alphan (ORCID 0000-0003-4391-0189 & YÖK ID 23851), Mohammad Hadi, Layla, Yaghini, Elnaz, Loizidou, Marilena, MacRobert, Alexander J., Koç University Surface Science and Technology Center (KUYTAM) / Koç Üniversitesi Yüzey Teknolojileri Araştırmaları Merkezi (KUYTAM), College of Sciences, Graduate School of Sciences and Engineering, Department of Chemistry, Department of Materials Science and Engineering, Department of Physics |
| المصدر: | Nanoscale |
| بيانات النشر: | Royal Society of Chemistry (RSC) International |
| سنة النشر: | 2021 |
| المجموعة: | Koç University Suna Kıraç Library’ Digital Collections |
| مصطلحات موضوعية: | Chemistry, Nanoscience and nanotechnology, Materials science, Physics, Science and technology, Aminolevulinic Acid, Photodynamic therapy, Actinic keratosis |
| الوصف: | Colorectal cancer (CRC) has a poor prognosis and urgently needs better therapeutic approaches. 5-Aminolevulinic acid (ALA) induced protoporphyrin IX (PpIX) based photodynamic therapy (PDT) is already used in the clinic for several cancers but not yet well investigated for CRC. Currently, systemic administration of ALA offers a limited degree of tumour selectivity, except for intracranial tumours, limiting its wider use in the clinic. The combination of effective ALA-PDT and chemotherapy may provide a promising alternative approach for CRC treatment. Herein, theranostic Ag2S quantum dots (AS-2MPA) optically trackable in near-infrared (NIR), conjugated with endothelial growth factor receptor (EGFR) targeting Cetuximab (Cet) and loaded with ALA for PDT monotherapy or ALA/5-fluorouracil (5FU) for the combination therapy are proposed for enhanced treatment of EGFR(+) CRC. AS-2MPA-Cet exhibited excellent targeting of the high EGFR expressing cells and showed a strong intracellular signal for NIR optical detection in a comparative study performed on SW480, HCT116, and HT29 cells, which exhibit high, medium and low EGFR expression, respectively. Targeting provided enhanced uptake of the ALA loaded nanoparticles by strong EGFR expressing cells and formation of higher levels of PpIX. Cells also differ in their efficiency to convert ALA to PpIX, and SW480 was the best, followed by HT29, while HCT116 was determined as unsuitable for ALA-PDT. The therapeutic efficacy was evaluated in 2D cell cultures and 3D spheroids of SW480 and HT29 cells using AS-2MPA with either electrostatically loaded, hydrazone or amide linked ALA to achieve different levels of pH or enzyme sensitive release. Most effective phototoxicity was observed in SW480 cells using AS-2MPA-ALA-electrostatic-Cet due to enhanced uptake of the particles, fast ALA release and effective ALA-to-PpIX conversion. Targeted delivery reduced the effective ALA concentration significantly which was further reduced with codelivery of 5FU. Delivery of ALA via covalent linkages ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | |
| اللغة: | English |
| تدمد: | 2040-3364 2040-3372 |
| العلاقة: | Publisher version; Koç University Institutional Repository; Hashemkhani, M, G Demirci, A Bayir, A Muti, A Sennaroglu, Hadi L. Mohammad, E Yaghini, M Loizidou, AJ MacRobert, and Acar H. Yagci. ""Cetuximab-ag2s Quantum Dots for Fluorescence Imaging and Highly Effective Combination of Ala-Based Photodynamic/chemo-Therapy of Colorectal Cancer Cells."" Nanoscale. 13.35 (2021): 14879-14899.; https://dx.doi.org/10.1039/d1nr03507jTest; IR03187.pdf; WoS; Scopus; PubMed; 216Z131; 334995; TÜBİTAK; http://libdigitalcollections.ku.edu.tr/cdm/ref/collection/IR/id/9949Test |
| DOI: | 10.1039/d1nr03507j |
| الإتاحة: | https://doi.org/10.1039/d1nr03507jTest http://libdigitalcollections.ku.edu.tr/cdm/ref/collection/IR/id/9949Test |
| رقم الانضمام: | edsbas.8939A1DF |
| قاعدة البيانات: | BASE |
| تدمد: | 20403364 20403372 |
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| DOI: | 10.1039/d1nr03507j |