دورية أكاديمية
Differential stability of beta-catenin along the animal-vegetal axis of the sea urchin embryo mediated by dishevelled.
| العنوان: | Differential stability of beta-catenin along the animal-vegetal axis of the sea urchin embryo mediated by dishevelled. |
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| المؤلفون: | Heather E. Weitzel, Michele R. Illies, Christine A. Byrum, Ronghui Xu, Athula H. Wikramanayake, Charles A. Ettensohn |
| سنة النشر: | 2018 |
| المجموعة: | KiltHub Research from Carnegie Mellon University |
| مصطلحات موضوعية: | Biological Sciences not elsewhere classified, Adaptor Proteins, Signal Transducing, Animals, Body Patterning, Cloning, Molecular, Cytoskeletal Proteins, Embryo, Nonmammalian, Glycogen Synthase Kinase 3, Kinetics, Phosphoproteins, Recombinant Proteins, Sea Urchins, Signal Transduction, Trans-Activators, beta Catenin |
| الوصف: | beta-Catenin has a central role in the early axial patterning of metazoan embryos. In the sea urchin, beta-catenin accumulates in the nuclei of vegetal blastomeres and controls endomesoderm specification. Here, we use in-vivo measurements of the half-life of fluorescently tagged beta-catenin in specific blastomeres to demonstrate a gradient in beta-catenin stability along the animal-vegetal axis during early cleavage. This gradient is dependent on GSK3beta-mediated phosphorylation of beta-catenin. Calculations show that the difference in beta-catenin half-life at the animal and vegetal poles of the early embryo is sufficient to produce a difference of more than 100-fold in levels of the protein in less than 2 hours. We show that dishevelled (Dsh), a key signaling protein, is required for the stabilization of beta-catenin in vegetal cells and provide evidence that Dsh undergoes a local activation in the vegetal region of the embryo. Finally, we report that GFP-tagged Dsh is targeted specifically to the vegetal cortex of the fertilized egg. During cleavage, Dsh-GFP is partitioned predominantly into vegetal blastomeres. An extensive mutational analysis of Dsh identifies several regions of the protein that are required for vegetal cortical targeting, including a phospholipid-binding motif near the N-terminus. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/Differential_stability_of_beta-catenin_along_the_animal-vegetal_axis_of_the_sea_urchin_embryo_mediated_by_dishevelled_/6097727Test |
| DOI: | 10.1184/r1/6097727.v1 |
| الإتاحة: | https://doi.org/10.1184/r1/6097727.v1Test https://figshare.com/articles/Differential_stability_of_beta-catenin_along_the_animal-vegetal_axis_of_the_sea_urchin_embryo_mediated_by_dishevelled_/6097727Test |
| حقوق: | In Copyright |
| رقم الانضمام: | edsbas.260F441B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1184/r1/6097727.v1 |
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