دورية أكاديمية
أعرض في EDS

A critical role for miR-142 in alveolar epithelial lineage formation in mouse lung development

التفاصيل البيبلوغرافية
العنوان: A critical role for miR-142 in alveolar epithelial lineage formation in mouse lung development
المؤلفون: Shrestha A., Carraro G., Nottet N., Vazquez-Armendariz A., Herold S., Cordero J., Singh I., Wilhelm J., Barreto G., Morty R., El Agha E., Mari B., Chen C., Zhang J., Chao C., Bellusci S.
المصدر: SCOPUS1420682X-2019-SID85063111227
سنة النشر: 2019
المجموعة: Kazan Federal University Digital Repository
مصطلحات موضوعية: Alveolar epithelium, Beta-catenin, EP300, Lung, microRNA-142
الوصف: © 2019, Springer Nature Switzerland AG. The respiratory epithelium arises from alveolar epithelial progenitors which differentiate into alveolar epithelial type 1 (AT1) and type 2 (AT2) cells. AT2 cells are stem cells in the lung critical for the repair process after injury. Mechanisms regulating AT1 and AT2 cell maturation are poorly defined. We report that the activation of the glucocorticoid pathway in an in vitro alveolar epithelial lineage differentiation assay led to increased AT2 marker Sftpc and decreased miR-142 expression. Using miR-142 KO mice, we demonstrate an increase in the AT2/AT1 cell number ratio. Overexpression of miR-142 in alveolar progenitor cells in vivo led to the opposite effect. Examination of the KO lungs at E18.5 revealed enhanced expression of miR-142 targets Apc, Ep300 and Kras associated with increased β-catenin and p-Erk signaling. Silencing of miR-142 expression in lung explants grown in vitro triggers enhanced Sftpc expression as well as increased AT2/AT1 cell number ratio. Pharmacological inhibition of Ep300-β-catenin but not Erk in vitro prevented the increase in Sftpc expression triggered by loss of miR-142. These results suggest that the glucocorticoid-miR-142-Ep300-β-catenin signaling axis controls pneumocyte maturation.
نوع الوثيقة: article in journal/newspaper
اللغة: unknown
تدمد: 1420-682X
العلاقة: Cellular and Molecular Life Sciences; http://dspace.kpfu.ru/xmlui/bitstream/net/155984/-1/SCOPUS1420682X-2019-SID85063111227-p1.pdfTest; https://dspace.kpfu.ru/xmlui/handle/net/155984Test
الإتاحة: https://dspace.kpfu.ru/xmlui/handle/net/155984Test
رقم الانضمام: edsbas.CFA6074
قاعدة البيانات: BASE
الوصف
تدمد:1420682X