دورية أكاديمية
Membrane-type 1 matrix metalloproteinase regulates fibronectin assembly and N-cadherin adhesion
| العنوان: | Membrane-type 1 matrix metalloproteinase regulates fibronectin assembly and N-cadherin adhesion |
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| المؤلفون: | Takino Takahisa, Yoshimoto Taisuke, Nakada Mitsutoshi, Li Zichen, Domoto Takahiro, Kawashiri Shuici, Sato Hiroshi, 滝野 隆久, 中田 光俊, 堂本 貴寛, 川尻 秀一, 佐藤 博 |
| بيانات النشر: | Academic Press |
| سنة النشر: | 2014 |
| المجموعة: | Kanazawa University Repository for Academic Resources (KURA) / 金沢大学学術情報リポジトリ |
| مصطلحات موضوعية: | Cell-matrix adhesion, Fibronectin, MT1-MMP, N-cadherin, Tumor |
| الوصف: | Fibronectin matrix formation requires the increased cytoskeletal tension generated by cadherin adhesions, and is suppressed by membrane-type 1 matrix metalloproteinase (MT1-MMP). In a co-culture of Rat1 fibroblasts and MT1-MMP-silenced HT1080 cells, fibronectin fibrils extended from Rat1 to cell-matrix adhesions in HT1080 cells, and N-cadherin adhesions were formed between Rat1 and HT1080 cells. In control HT1080 cells contacting with Rat1 fibroblasts, cell-matrix adhesions were formed in the side away from Rat1 fibroblasts, and fibronectin assembly and N-cadherin adhesions were not formed. The role of N-cadherin adhesions in fibronectin matrix formation was studied using MT1-MMP-silenced HT1080 cells. MT1-MMP knockdown promoted fibronectin matrix assembly and N-cadherin adhesions in HT1080 cells, which was abrogated by double knockdown with either integrin β1 or fibronectin. Conversely, inhibition of N-cadherin adhesions by its knockdown or treatment with its neutralizing antibody suppressed fibronectin matrix formation in MT1-MMP-silenced cells. These results demonstrate that fibronectin assembly initiated by MT1-MMP knockdown results in increase of N-cadherin adhesions, which are prerequisite for further fibronectin matrix formation. © 2014 Elsevier Inc. All rights reserved. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1090-2104 |
| العلاقة: | https://kanazawa-u.repo.nii.ac.jp/?action=repository_uri&item_id=14077Test; http://hdl.handle.net/2297/39702Test; Biochemical and Biophysical Research Communications, 450(2), 1016-1020(2014-07-25); AA11542044; https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=14077&item_no=1&attribute_id=26&file_no=1Test |
| الإتاحة: | http://hdl.handle.net/2297/39702Test https://kanazawa-u.repo.nii.ac.jp/?action=repository_uri&item_id=14077Test https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=14077&item_no=1&attribute_id=26&file_no=1Test |
| رقم الانضمام: | edsbas.292C70BA |
| قاعدة البيانات: | BASE |
| تدمد: | 10902104 |
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