دورية أكاديمية
Functional cardiomyocytes derived from Isl1 cardiac progenitors via Bmp4 stimulation
| العنوان: | Functional cardiomyocytes derived from Isl1 cardiac progenitors via Bmp4 stimulation |
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| المؤلفون: | Çağavi, Esra, Bartulos, Oscar, Suh, Carol Young, Sun, Baonan, Yue, Zhichao, Jiang, Zhengxin, Yue, Lixia, Qyang, Yibing |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2014 |
| المجموعة: | İstanbul Medipol University Institutional Repository (DSpace@Medipol) |
| مصطلحات موضوعية: | Functional Cardiomyocytes, Isl1, Bmp4 |
| الوصف: | WOS: 000348845600001 ; PubMed ID: 25522363 ; As heart failure due to myocardial infarction remains a leading cause of morbidity worldwide, cell-based cardiac regenerative therapy using cardiac progenitor cells (CPCs) could provide a potential treatment for the repair of injured myocardium. As adult CPCs may have limitations regarding tissue accessibility and proliferative ability, CPCs derived from embryonic stem cells (ESCs) could serve as an unlimited source of cells with high proliferative ability. As one of the CPCs that can be derived from embryonic stem cells, Isl1 expressing cardiac progenitor cells (Isl1-CPCs) may serve as a valuable source of cells for cardiac repair due to their high cardiac differentiation potential and authentic cardiac origin. In order to generate an unlimited number of Isl1-CPCs, we used a previously established an ESC line that allows for isolation of Isl1-CPCs by green fluorescent protein (GFP) expression that is directed by the mef2c gene, specifically expressed in the Isl1 domain of the anterior heart field. To improve the efficiency of cardiac differentiation of Isl1-CPCs, we studied the role of Bmp4 in cardiogenesis of Isl1-CPCs. We show an inductive role of Bmp directly on cardiac progenitors and its enhancement on early cardiac differentiation of CPCs. Upon induction of Bmp4 to Isl1-CPCs during differentiation, the cTnT+ cardiomyocyte population was enhanced 2.8 +/- 0.4 fold for Bmp4 treated CPC cultures compared to that detected for vehicle treated cultures. Both Bmp4 treated and untreated cardiomyocytes exhibit proper electrophysiological and calcium signaling properties. In addition, we observed a significant increase in Tbx5 and Tbx20 expression in differentiation cultures treated with Bmp4 compared to the untreated control, suggesting a link between Bmp4 and Tbx genes which may contribute to the enhanced cardiac differentiation in Bmp4 treated cultures. Collectively these findings suggest a cardiomyogenic role for Bmp4 directly on a pure population of Isl1 expressing ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| العلاقة: | PLoS One; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; Çağavi, E., Bartulos, O., Suh, C. Y., Sun, B., Yue, Z., Jiang, Z. . Qyang, Y. (2014). Functional cardiomyocytes derived from Isl1 cardiac progenitors via Bmp4 stimulation. PLoS One, 9(12). https://dx.doi.org/10.1371/journal.pone.0110752Test; https://dx.doi.org/10.1371/journal.pone.0110752Test; https://hdl.handle.net/20.500.12511/3692Test; 12; Q2; Q1 |
| DOI: | 10.1371/journal.pone.0110752 |
| الإتاحة: | https://doi.org/20.500.12511/3692Test https://doi.org/10.1371/journal.pone.0110752Test https://hdl.handle.net/20.500.12511/3692Test |
| حقوق: | info:eu-repo/semantics/openAccess ; Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.4854CA7C |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0110752 |