المؤلفون: Senna G. W., Dantas E. H. M., Scudese E., Brandao P. P., Lira V. A., Baffi M., Ribeiro L. C. P., Simao R., Thomas E., Bianco A.

المساهمون: Senna G.W., Dantas E.H.M., Scudese E., Brandao P.P., Lira V.A., Baffi M., Ribeiro L.C.P., Simao R., Thomas E., Bianco A.

مصطلحات موضوعية: creatine kinase, cytokines, L-Lactate Dehydrogenase, leukocytes, physical fitness, resistance training

الوصف: Objectives: The aim of the manuscript was to analyze the effects of two rest periods between volume-equated resistance exercise (RE) on inflammatory responses (cytokines and leukocyte) and muscle damage. Methods: Ten trained men (26.40 ± 4.73 years, 80.71 ± 8.95 kg, and 176.03 ± 6.11 cm) voluntarily participated in training sessions consisting of five sets of 10 reps performed at 10-RM on (1) the barbell bench press followed by (2) leg press, with either 1- or 3-min rest between sets and exercises. Circulating concentrations of different biomarkers was measured before (Pre), and after 3 h (excepted for cytokines), 6, 12, and 24 h from exercise. The rate of perceived exertion (RPE) was recorded after each set on both planned visits. Results: We found greater increases triggered by the 1-min rest period in Creatine Kinase (CK), occurring from 12 to 24 h post-exercise compared to the 3-min rest condition. A significant increase in the 1-min rest condition was also observed in the total number of leukocytes, neutrophils, and monocytes. The 1-min rest period also triggered increases compared to baseline in pro-inflammatory cytokines [Interleukin 1 beta (IL-1β), p = 0.004; tumor necrosis factor α (TNF-α), p = 0.01; and granulocyte-macrophage colony-stimulating factor (GM-CSF), p = 0.01], which were more evident after 6 and 12 h post-exercise. Similarly, increases in anti-inflammatory cytokines [Interleukin 5 (IL-5), p = 0.01; Interleukin 6 (IL-6), p = 0.01; and Interleukin 10 (IL-10), p = 0.01] at all time-points were observed. Conclusion: Our results indicate that a 1-min rest condition in volume-equated RE promoted greater overall muscle tissue damage with a longer duration of the inflammatory processes compared to a 3-min rest.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35295573; info:eu-repo/semantics/altIdentifier/wos/WOS:000772682800001; volume:13; firstpage:827847; numberofpages:8; journal:FRONTIERS IN PHYSIOLOGY; https://hdl.handle.net/10447/579076Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85127054900

الإتاحة: https://doi.org/10.3389/fphys.2022.827847Test
https://hdl.handle.net/10447/579076Test

المؤلفون: Giovanna Li Petri, Btissame El Hassouni, Rocco Sciarrillo, Niccola Funel, Giulia Mantini, Eveline A. Zeeuw van der Laan, Stella Cascioferro, Amir Avan, Paolo Andrea Zucali, Nadia Zaffaroni, Tonny Lagerweij, Barbara Parrino, Kees Smid, Marcello Deraco, Carlotta Granchi, Alicja Braczko, Ryszard T. Smolenski, Larry H. Matherly, Gerrit Jansen, Yehuda G. Assaraf, Patrizia Diana, Jacqueline Cloos, Godefridus J. Peters, Filippo Minutolo, Elisa Giovannetti

المساهمون: Giovanna Li Petri, Btissame El Hassouni, Rocco Sciarrillo, Niccola Funel, Giulia Mantini, Eveline A. Zeeuw van der Laan, Stella Cascioferro, Amir Avan, Paolo Andrea Zucali, Nadia Zaffaroni, Tonny Lagerweij, Barbara Parrino, Kees Smid, Marcello Deraco, Carlotta Granchi, Alicja Braczko, Ryszard T. Smolenski, Larry H. Matherly, Gerrit Jansen, Yehuda G. Assaraf, Patrizia Diana, Jacqueline Cloo, Godefridus J. Peter, Filippo Minutolo, Elisa Giovannetti

مصطلحات موضوعية: proton-coupled folate transporter, mesothelioma, hypoxia, lactate dehydrogenase inhibitors, Settore CHIM/08 - Chimica Farmaceutica

الوصف: BACKGROUND: Expression of proton-coupled folate transporter (PCFT) is associated with survival of mesothelioma patients treated with pemetrexed, and is reduced by hypoxia, prompting studies to elucidate their correlation. METHODS: Modulation of glycolytic gene expression was evaluated by PCR arrays in tumour cells and primary cultures growing under hypoxia, in spheroids and after PCFT silencing. Inhibitors of lactate dehydrogenase (LDH-A) were tested in vitro and in vivo. LDH-A expression was determined in tissue microarrays of radically resected malignant pleural mesothelioma (MPM, N = 33) and diffuse peritoneal mesothelioma (DMPM, N = 56) patients. RESULTS: Overexpression of hypoxia marker CAIX was associated with low PCFT expression and decreased MPM cell growth inhibition by pemetrexed. Through integration of PCR arrays in hypoxic cells and spheroids and following PCFT silencing, we identified the upregulation of LDH-A, which correlated with shorter survival of MPM and DMPM patients. Novel LDH-A inhibitors enhanced spheroid disintegration and displayed synergistic effects with pemetrexed in MPM and gemcitabine in DMPM cells. Studies with bioluminescent hypoxic orthotopic and subcutaneous DMPM athymic-mice models revealed the marked antitumour activity of the LDH-A inhibitor NHI-Glc-2, alone or combined with gemcitabine. CONCLUSIONS: This study provides novel insights into hypoxia/PCFT-dependent chemoresistance, unravelling the potential prognostic value of LDH-A, and demonstrating the preclinical activity of LDH-A inhibitors.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/32493992; info:eu-repo/semantics/altIdentifier/wos/WOS:000537679900001; volume:123; issue:4; firstpage:644; lastpage:656; numberofpages:13; journal:BRITISH JOURNAL OF CANCER; http://hdl.handle.net/10447/453940Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85085931027

الإتاحة: https://doi.org/10.1038/s41416-020-0912-9Test
http://hdl.handle.net/10447/453940Test

المؤلفون: Agnello, Luisa, Buscemi, Salvatore, Di Buono, Giuseppe, Vidali, Matteo, Lo Sasso, Bruna, Agrusa, Antonino, Ciaccio, Marcello

المساهمون: Agnello, Luisa, Buscemi, Salvatore, Di Buono, Giuseppe, Vidali, Matteo, Lo Sasso, Bruna, Agrusa, Antonino, Ciaccio, Marcello

مصطلحات موضوعية: anastomotic leakage, surgery, C-reactive protein (CRP), lactate dehydrogenase (LDH), neutrophil to lymphocyte ratio (NLR), biomarkers

الوصف: Objectives In this study, we investigated the role of several circulating and drainage fluid biomarkers for detecting postoperative complications (PCs) and anastomotic leakage (AL) in patients undergoing colorectal surgery. Methods All consecutive patients undergoing colorectal surgery between June 2018 and April 2020 were prospectively considered. On postoperative days (POD) 1, 3, and 5, we measured lactate dehydrogenase (LDH) in drainage fluid, C-reactive protein (CRP) in serum and drainage fluid, and neutrophil to lymphocyte ratio (NLR). Results We enrolled 187 patients. POD1 patients with AL had higher serum CRP levels, while on POD3 and on POD5 higher NLR and serum CRP. LDH and CRP in drainage fluid were also significantly higher at both time points. The area under the curves (AUCs) of serum and drainage fluid CRP were 0.752 (0.629–0.875) and 0.752 (0.565–0.939), respectively. The best cut-off for serum and drainage fluid CRP was 185.23 and 76 mg/dL, respectively. The AUC of NLR on POD3 was 0.762 (0.662–0.882) with a sensitivity and specificity of 84 and 63 %, respectively, at a cut-off of 6,6. Finally, drainage fluid LDH showed the best diagnostic performance for AL, with an AUC, sensitivity, and specificity of 0.921 (0.849–0.993), 82 %, and 90 % at a cut-off of 2,186 U/L. Trends in serum parameters between patients with or without PCs or AL were also evaluated. Interestingly, we found that NLR decreased faster in patients without PCs than in patients with PCs and patients with AL. Conclusions Drainage fluid LDH and NLR could be promising biomarkers of PCs and AL.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/37988156; info:eu-repo/semantics/altIdentifier/wos/WOS:001112184200001; volume:0; issue:0; numberofpages:12; journal:CLINICAL CHEMISTRY AND LABORATORY MEDICINE; https://hdl.handle.net/10447/626013Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85177793649

الإتاحة: https://doi.org/10.1515/cclm-2023-1164Test
https://hdl.handle.net/10447/626013Test

المؤلفون: LI PETRI, Giovanna

المساهمون: DIANA, Patrizia

مصطلحات موضوعية: imidazo[2,1-b][1,3,4]thiadiazole derivative, pancreatic ductal adenocarcinoma, antiproliferative activity, inhibition of migration, spheroids shrinkage, modulation of EMT, PTK2/FAK, malignant pleural and peritoneal mesothelioma, proton-coupled folate transporter, pemetrexed, chemoresistance, hypoxia, lactate dehydrogenase, xenograft, prognosi, anti-biofilm agent, Anti-virulence agent, Staphylococcal biofilm inhibitor, Settore CHIM/08 - Chimica Farmaceutica

العلاقة: http://hdl.handle.net/10447/395253Test

الإتاحة: http://hdl.handle.net/10447/395253Test

المؤلفون: PROIA, Patrizia, BIANCO, Antonino, SCHIERA, Gabriella, SALADINO, Patrizia, PETRUCCI, Marco, TRAINA, Marcello, PALMA, Antonio, Pomara, F

المساهمون: Proia, P, Bianco, A, Schiera, G, Saladino, P, Pomara, F, Petrucci, M, Traina, M, Palma, A

مصطلحات موضوعية: creatine kinase, lactate dehydrogenase, PPAR alpha, PPAR gamma, Settore BIO/10 - Biochimica

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:000303267300014; volume:52; issue:1; firstpage:102; lastpage:106; numberofpages:5; journal:THE JOURNAL OF SPORTS MEDICINE AND PHYSICAL FITNESS; http://hdl.handle.net/10447/97971Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84860129277

الإتاحة: http://hdl.handle.net/10447/97971Test