دورية أكاديمية
G-Quadruplex Recognition by DARPIns through Epitope/Paratope Analogy**
| العنوان: | G-Quadruplex Recognition by DARPIns through Epitope/Paratope Analogy** |
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| المؤلفون: | Miclot T., Bignon E., Terenzi A., Grandemange S., Barone G., Monari A. |
| المساهمون: | Miclot T., Bignon E., Terenzi A., Grandemange S., Barone G., Monari A. |
| بيانات النشر: | John Wiley and Sons Inc |
| سنة النشر: | 2022 |
| المجموعة: | IRIS Università degli Studi di Palermo |
| مصطلحات موضوعية: | c-Myc promoter, DARPin, epitope/paratope recognition, guanine quadruplex, molecular dynamics, Settore CHIM/03 - Chimica Generale E Inorganica |
| الوصف: | We investigated the mechanisms leading to the specific recognition of Guanine Guadruplex (G4) by DARPins peptides, which can lead to the design of G4s specific sensors. To this end we carried out all-atom molecular dynamics simulations to unravel the interactions between specific nucleic acids, including human-telomeric (h-telo), Bcl-2, and c-Myc, with different peptides, forming a DARPin/G4 complex. By comparing the sequences of DARPin with that of a peptide known for its high affinity for c-Myc, we show that the recognition cannot be ascribed to sequence similarity but, instead, depends on the complementarity between the three-dimensional arrangement of the molecular fragments involved: the α-helix/loops domain of DARPin and the G4 backbone. Our results reveal that DARPins tertiary structure presents a charged hollow region in which G4 can be hosted, thus the more complementary the structural shapes, the more stable the interaction. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000842811600001; volume:28; issue:57; numberofpages:11; journal:CHEMISTRY-A EUROPEAN JOURNAL; https://hdl.handle.net/10447/572368Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85136477951 |
| DOI: | 10.1002/chem.202201824 |
| الإتاحة: | https://doi.org/10.1002/chem.202201824Test https://hdl.handle.net/10447/572368Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.CE7BDE0F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/chem.202201824 |
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