دورية أكاديمية
Heat shock protein (Hsp) regulation by muscarinic acetylcholine receptor (mAChR) activation in the rat hippocampus
| العنوان: | Heat shock protein (Hsp) regulation by muscarinic acetylcholine receptor (mAChR) activation in the rat hippocampus |
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| المؤلفون: | Frinchi, Monica, SCADUTO, Pietro, Cappello, Francesco, Belluardo, Natale, Mudò, Giuseppa |
| المساهمون: | Frinchi, Monica, Scaduto, Pietro, Cappello, Francesco, Belluardo, Natale, Mudò, Giuseppa* |
| بيانات النشر: | Wiley-Liss Inc. |
| سنة النشر: | 2018 |
| المجموعة: | IRIS Università degli Studi di Palermo |
| مصطلحات موضوعية: | heat shock factor 1, heat shock protein, Hsp70, Hsp90, muscarinic receptor, Physiology, Clinical Biochemistry, Cell Biology |
| الوصف: | The cholinergic system plays a crucial role in modulating in the central nervous system physiological responses such as neurogenesis, neuronal differentiation, synaptic plasticity, and neuroprotection. In a recent study, we showed that Oxotremorine-M, a non-selective muscarinic acetylcholine receptor agonist, is able to transactivate the fibroblast growth factor receptor and to produce a significant increase in the hippocampal primary neurite outgrowth. In the present study we aimed to explore in the rat hippocampus the possible effect of acute or chronic treatment with Oxotremorine-M on some heat shock proteins (Hsp60, Hsp70, Hsp90) and on activation of related transcription factor heat shock factor 1 (HSF1). Following single injection of Oxotremorine-M (0.4 mg/kg) all Hsps examined were significantly increased in at least one of the time points studied (24, 48, and 72 hr). Treatment with Oxotremorine-M significantly increased the level of phosphorylated HSF1 in all time points studied, without change of protein levels. Similar pattern of Hsps changes was obtained following chronic Oxotremorine-M treatment (0.2 mg/kg) for 5 days. Surprisingly, following chronic treatment for 10 days no changes were observed in Hsps. The muscarinic acetylcholine receptor antagonist scopolamine (1 mg/kg) was able to completely block Oxotremorine-M effects on Hsps. In conclusion, considering the function of Hsps in protecting neuronal cells from deleterious proteotoxic stress, for example, protein mis-folding and aggregation, the results obtained indicate that muscarinic acetylcholine receptor activation may have implications in potential treatment of neurodegenerative disorders linked to protein aggregation, such as Alzheimer disease. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/29323700; info:eu-repo/semantics/altIdentifier/wos/WOS:000430797600054; volume:233; issue:8; firstpage:6107; lastpage:6116; numberofpages:10; journal:JOURNAL OF CELLULAR PHYSIOLOGY; http://hdl.handle.net/10447/295439Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85043333943; http://onlinelibrary.wiley.com/journal/10.1002Test/(ISSN)1097-4652 |
| DOI: | 10.1002/jcp.26454 |
| DOI: | 10.1002/(ISSN)1097-4652 |
| الإتاحة: | https://doi.org/10.1002/jcp.26454Test http://hdl.handle.net/10447/295439Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.FEA6BA39 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/jcp.26454 |
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