دورية أكاديمية
Tunneling nanotubes spread fibrillar α-synuclein by intercellular trafficking of lysosomes
| العنوان: | Tunneling nanotubes spread fibrillar α-synuclein by intercellular trafficking of lysosomes |
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| المؤلفون: | Abounit S., Bousset L., Loria F., Zhu S., de Chaumont F., Pieri L., Olivo-Marin J., Melki R., Zurzolo C. |
| المساهمون: | Abounit, S., Bousset, L., Loria, F., Zhu, S., de Chaumont, F., Pieri, L., Olivo-Marin, J., Melki, R., Zurzolo, C. |
| سنة النشر: | 2016 |
| المجموعة: | IRIS Università degli Studi di Napoli Federico II |
| مصطلحات موضوعية: | intercellular transfer, synucleinopathie, TNT, α-synuclein, Amyloid, Animal, Cells, Cultured, Coculture Technique, Lysosome, Mice, Microscopy, Fluorescence, Neuron, alpha-Synuclein, Cell Communication, Nanotubes |
| الوصف: | Synucleinopathies such as Parkinson's disease are characterized by the pathological deposition of misfolded α-synuclein aggregates into inclusions throughout the central and peripheral nervous system. Mounting evidence suggests that intercellular propagation of α-synuclein aggregates may contribute to the neuropathology; however, the mechanism by which spread occurs is not fully understood. By using quantitative fluorescence microscopy with co-cultured neurons, here we show that α-synuclein fibrils efficiently transfer from donor to acceptor cells through tunneling nanotubes (TNTs) inside lysosomal vesicles. Following transfer through TNTs, α-synuclein fibrils are able to seed soluble α-synuclein aggregation in the cytosol of acceptor cells. We propose that donor cells overloaded with α-synuclein aggregates in lysosomes dispose of this material by hijacking TNT-mediated intercellular trafficking. Our findings thus reveal a possible novel role of TNTs and lysosomes in the progression of synucleinopathies. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000385707500007; volume:35; issue:19; firstpage:2120; lastpage:2138; numberofpages:19; journal:EMBO JOURNAL; http://hdl.handle.net/11588/816239Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84989908457 |
| DOI: | 10.15252/embj.201593411 |
| الإتاحة: | https://doi.org/10.15252/embj.201593411Test http://hdl.handle.net/11588/816239Test |
| رقم الانضمام: | edsbas.ADA5A598 |
| قاعدة البيانات: | BASE |
| DOI: | 10.15252/embj.201593411 |
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