التفاصيل البيبلوغرافية
| العنوان: |
The human-immunodeficiency-virus type-1 Long Terminal Repeat is activated by monofunctional and bifunctional DNA alkylating-agents in human-lymphocytes. |
| المؤلفون: |
I. QUINTO, M. R. RUOCCO, F. BALDASSARRE, M. MALLARDO, E. DRAGONETTI, G. SCALA |
| المساهمون: |
Quinto, I., Ruocco, M. R., Baldassarre, F., Mallardo, M., Dragonetti, E., Scala, G. |
| سنة النشر: |
1993 |
| المجموعة: |
IRIS Università degli Studi di Napoli Federico II |
| مصطلحات موضوعية: |
HIV-1, LTR, phorbol 12-myristate 13-acetate, methylmethane sulfonate |
| الوصف: |
The activation of the human immunodeficiency virus, type 1 (HIV-1) by the DNA alkylating agents ethyl methanesulfonate, methyl methanesulfonate, and mitomycin C was observed in human B lymphocytes transiently transfected with plasmids in which the HIV-1 long terminal repeat (LTR) directed the expression of the bacterial chloramphenicol acetyltransferase gene. Deletion of the two NF-kappa B-binding sites of LTR abolished the HIV-1 activation induced by the three mutagens, while deletion of the three Sp1-binding sites slightly reduced it. Electrophoretic mobility shift assays revealed an increased binding to the kappa B sites of HIV-1 LTR in the nuclear extracts of human B lymphocytes upon mutagen treatment, while binding to Sp1 sites was unaffected. The TAR region was also involved in the mutagen-mediated activation of HIV-1 LTR inasmuch as a small deletion in the TAR sequence (nucleotides +34 to +37) greatly decreased the induction of HIV-1 expression. Moreover, an enhanced binding activity to the TAR DNA sequence (nucleotides +24 to +47) was observed in nuclear extracts of mutagen-treated lymphocytes. Thus, both the enhancer and the 5'-untranslated region of HIV-1 functionally cooperate in the mutagen-mediated induction of HIV-1 expression. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
STAMPA |
| اللغة: |
English |
| العلاقة: |
info:eu-repo/semantics/altIdentifier/wos/WOS:A1993MK42500103; volume:268; issue:35; firstpage:26719; lastpage:26724; numberofpages:6; journal:THE JOURNAL OF BIOLOGICAL CHEMISTRY; http://hdl.handle.net/11588/159493Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0027527553 |
| الإتاحة: |
http://hdl.handle.net/11588/159493Test |
| حقوق: |
info:eu-repo/semantics/closedAccess |
| رقم الانضمام: |
edsbas.39FE3FE0 |
| قاعدة البيانات: |
BASE |
