دورية أكاديمية
Serological Assays for Alveolar and Cystic Echinococcosis-A Comparative Multi-Test Study in Switzerland and Kyrgyzstan
| العنوان: | Serological Assays for Alveolar and Cystic Echinococcosis-A Comparative Multi-Test Study in Switzerland and Kyrgyzstan |
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| المؤلفون: | Kronenberg, Philipp A, Deibel, Ansgar, Gottstein, Bruno, Grimm, Felix, Müllhaupt, Beat, Meyer Zu Schwabedissen, Cordula, Aitbaev, Sezdbek, Omorov, Rakhatbek A, Abdykerimov, Kubanychbek K, Minbaeva, Gulnara, Usubalieva, Jumagul, Siles-Lucas, Mar, Pepe, Paola, Rinaldi, Laura, Spiliotis, Markus, Wang, Junhua, Müller, Norbert, Torgerson, Paul R, Deplazes, Peter |
| المساهمون: | Kronenberg, Philipp A, Deibel, Ansgar, Gottstein, Bruno, Grimm, Felix, Müllhaupt, Beat, Meyer Zu Schwabedissen, Cordula, Aitbaev, Sezdbek, Omorov, Rakhatbek A, Abdykerimov, Kubanychbek K, Minbaeva, Gulnara, Usubalieva, Jumagul, Siles-Lucas, Mar, Pepe, Paola, Rinaldi, Laura, Spiliotis, Marku, Wang, Junhua, Müller, Norbert, Torgerson, Paul R, Deplazes, Peter |
| سنة النشر: | 2022 |
| المجموعة: | IRIS Università degli Studi di Napoli Federico II |
| مصطلحات موضوعية: | ELISA, Echinococcus granulosus sensu lato, Echinococcus multiloculari, Western blot, antibodie, antigen, diagnosi, serology |
| الوصف: | Both alveolar (AE) and cystic echinococcosis (CE) are lacking pathognomonic clinical signs; consequently imaging technologies and serology remain the main pillars for diagnosis. The present study included 100 confirmed treatment-naïve AE and 64 CE patients that were diagnosed in Switzerland or Kyrgyzstan. Overall, 10 native Echinococcus spp. antigens, 3 recombinant antigens, and 4 commercial assays were comparatively evaluated. All native E. multilocularis antigens were produced in duplicates with a European and a Kyrgyz isolate and showed identical test values for the diagnosis of AE and CE. Native antigens and three commercial tests showed high diagnostic sensitivities (Se: 86-96%) and specificities (Sp: 96-99%) for the diagnosis of AE and CE in Swiss patients. In Kyrgyz patients, values of sensitivities and specificities were 10-20% lower as compared to the Swiss patients' findings. For the sero-diagnosis of AE in Kyrgyzstan, a test-combination of an E. multilocularis protoscolex antigen and the recombinant antigen Em95 appears to be the most suitable test strategy (Se: 98%, Sp: 87%). For the diagnosis of CE in both countries, test performances were hampered by major cross-reactions with AE patients and other parasitic diseases as well as by limited diagnostic sensitivities (93% in Switzerland and 76% in Kyrgyzstan, respectively). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000802403900001; volume:11; issue:5; firstpage:518; journal:PATHOGENS; http://hdl.handle.net/11588/895070Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85129701346 |
| DOI: | 10.3390/pathogens11050518 |
| الإتاحة: | https://doi.org/10.3390/pathogens11050518Test http://hdl.handle.net/11588/895070Test |
| رقم الانضمام: | edsbas.9154E879 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/pathogens11050518 |
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