دورية أكاديمية
Detection of Apoptotic Versus Autophagic Cell Death by Flow Cytometry
| العنوان: | Detection of Apoptotic Versus Autophagic Cell Death by Flow Cytometry |
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| المؤلفون: | Sica, Valentina, Kroemer, Guido, Galluzzi, Lorenzo, MAIURI, MARIA CHIARA |
| المساهمون: | Sica, Valentina, Maiuri, MARIA CHIARA, Kroemer, Guido, Galluzzi, Lorenzo |
| سنة النشر: | 2016 |
| المجموعة: | IRIS Università degli Studi di Napoli Federico II |
| مصطلحات موضوعية: | Autophagy, Immunogenic cell death, Mitochondrial outer membrane permeabilization, Mitochondrial permeability transition, Necroptosi, Necrosis |
| الوصف: | Different modes of regulated cell death (RCD) can be initiated by distinct molecular machineries and their morphological manifestations can be difficult to discriminate. Moreover, cells responding to stress often activate an adaptive response centered around autophagy, and whether such a response is cytoprotective or cytotoxic cannot be predicted based on morphological parameters only. Molecular definitions are therefore important to understand various RCD subroutines from a mechanistic perspective. In vitro, various forms of RCD including apoptosis and autophagic cell death can be easily discriminated from each other with assays that involve chemical or pharmacological interventions targeting key components of either pathway. Here, we detail a straightforward method to discriminate apoptosis from autophagic cell death by flow cytometry, based on the broad-spectrum caspase inhibitor Z-VAD-fmk and the genetic inhibition of ATG5. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000687297200002; volume:1419; firstpage:1; lastpage:16; numberofpages:16; journal:BIOCHEMISTRY; http://hdl.handle.net/11588/681039Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84964545003 |
| DOI: | 10.1007/978-1-4939-3581-9_1 |
| الإتاحة: | https://doi.org/10.1007/978-1-4939-3581-9_1Test http://hdl.handle.net/11588/681039Test |
| رقم الانضمام: | edsbas.4EC4A4E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/978-1-4939-3581-9_1 |
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