دورية أكاديمية
Microfluidic Chip With Integrated a-Si:H Photodiodes for Chemiluminescence-Based Bioassays
| العنوان: | Microfluidic Chip With Integrated a-Si:H Photodiodes for Chemiluminescence-Based Bioassays |
|---|---|
| المؤلفون: | Domenico Caputo, Giampiero de Cesare, Augusto Nascetti, Riccardo Scipinotti, DOLCI, LUISA STELLA, MIRASOLI, MARA, RODA, ALDO |
| المساهمون: | Domenico Caputo, Giampiero de Cesare, Luisa Stella Dolci, Mara Mirasoli, Augusto Nascetti, Aldo Roda, Riccardo Scipinotti |
| سنة النشر: | 2013 |
| المجموعة: | IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
| مصطلحات موضوعية: | Sensor systems and application, Chemiluminescence, Amorphous silicon sensors |
| الوصف: | On-chip optical detection of chemiluminescent reactions is presented. The device is based on the integration of thin film hydrogenated amorphous silicon photosensors on a functionalized glass substrate ensuring both a good optical coupling and an optimal separation between biological or chemical reagents and the sensing elements. The sensor has been characterized and optimized using the chemiluminescent system composed by the enzyme horseradish peroxidase (HRP) and luminol/peroxide/enhancer cocktail. The detectability of HRP is at the attomole level with a sensitivity of 1.46 fA/fg. Experiments, involving the detection of immobilized bio-specific probes on the functionalized surface have been performed both in bulk and microfluidics regime, proving the ability of the system to effectively detect chemiluminescent reactions and their kinetics. In particular, results achieved using conventional polydimethylsiloxane microfluidics for samples and reagents handling confirmed the good detection capabilities of the proposed system. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000320001100012; volume:13; firstpage:2595; lastpage:2602; numberofpages:8; journal:IEEE SENSORS JOURNAL; http://hdl.handle.net/11585/191372Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84878778133 |
| DOI: | 10.1109/JSEN.2013.2256889 |
| الإتاحة: | https://doi.org/10.1109/JSEN.2013.2256889Test http://hdl.handle.net/11585/191372Test |
| رقم الانضمام: | edsbas.60FE33AF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1109/JSEN.2013.2256889 |
|---|