دورية أكاديمية
Immunocytochemical detection of dentin matrix proteins in primary teeth from patients with dentinogenesis imperfecta associated with osteogenesis imperfecta
| العنوان: | Immunocytochemical detection of dentin matrix proteins in primary teeth from patients with dentinogenesis imperfecta associated with osteogenesis imperfecta |
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| المؤلفون: | Orsini, G, Majorana, A., Putignano, A., Polimeni, A., MAZZONI, ANNALISA, FALCONI, MIRELLA, BRESCHI, LORENZO |
| المساهمون: | Orsini, G, Majorana, A., Mazzoni, A., Putignano, A., Falconi, M., Polimeni, A., Breschi, Lorenzo |
| سنة النشر: | 2014 |
| المجموعة: | IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
| مصطلحات موضوعية: | Collagen, Dentinogenesis imperfecta, Immuno-electron microscopy, Non-collagenous protein, Osteogenesis imperfecta, Child, Collagen Type I, Extracellular Matrix Protein, Female, Human, Immunohistochemistry, Male, Osteopontin, Phosphoprotein, Dentin, Tooth, Deciduou, Cell Biology, Histology, Biophysics |
| الوصف: | Dentinogenesis imperfecta determines structural alterations of the collagen structure still not completely elucidated. Immunohistochemical analysis was used to assay Type I and VI collagen, various non-collagenous proteins distribution in human primary teeth from healthy patients or from patients affected by type I dentinogenesis imperfecta (DGI-I) associated with osteogenesis imperfecta (OI). In sound primary teeth, an organized well-known ordered pattern of the type I collagen fibrils was found, whereas atypical and disorganized fibrillar structures were observed in dentin of DGI-I affected patients. Expression of type I collagen was observed in both normal and affected primary teeth, although normal dentin stained more uniformly than DGI-I affected dentin. Reactivity of type VI collagen was significantly lower in normal teeth than in dentin from DGI-I affected patients (P<0.05). Expressions of dentin matrix protein (DMP)-1 and osteopontin (OPN) were observed in both normal dentin and dentin from DGI-I affected patients, without significant differences, being DMP1 generally more abundantly expressed. Immunolabeling for chondroitin sulfate (CS) and biglycan (BGN) was weaker in dentin from DGI-I-affected patients compared to normal dentin, this decrease being significant only for CS. This study shows ultrastructural alterations in dentin obtained from patients affected by DGI-I, supported by immunocytochemical assays of different collagenous and non-collagenous proteins. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/25578972; info:eu-repo/semantics/altIdentifier/wos/WOS:000347228000007; volume:58; issue:4; firstpage:301; lastpage:306; numberofpages:6; journal:EUROPEAN JOURNAL OF HISTOCHEMISTRY; http://hdl.handle.net/11585/586830Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84923062783; http://www.ejh.it/index.php/ejh/article/download/2405/2312Test |
| DOI: | 10.4081/ejh.2014.2405 |
| الإتاحة: | https://doi.org/10.4081/ejh.2014.2405Test http://hdl.handle.net/11585/586830Test http://www.ejh.it/index.php/ejh/article/download/2405/2312Test |
| رقم الانضمام: | edsbas.DDEF2B79 |
| قاعدة البيانات: | BASE |
| DOI: | 10.4081/ejh.2014.2405 |
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