دورية أكاديمية
Blastic plasmacytoid dendritic cell neoplasm: Genomics mark epigenetic dysregulation as a primary therapeutic target
| العنوان: | Blastic plasmacytoid dendritic cell neoplasm: Genomics mark epigenetic dysregulation as a primary therapeutic target |
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| المؤلفون: | Sapienza M. R., Abate F., Melle F., Orecchioni S., Fuligni F., Etebari M., Tabanelli V., Laginestra M. A., Pileri A., Motta G., Rossi M., Agostinelli C., Sabattini E., Pimpinelli N., Truni M., Falini B., Cerroni L., Talarico G., Piccioni R., Amente S., Indio V., Tarantino G., Brundu F., Paulli M., Berti E., Facchetti F., Dellino G. I., Bertolini F., Tripodo C., Rabadan R., Pileri S. A. |
| المساهمون: | Sapienza, M. R., Abate, F., Melle, F., Orecchioni, S., Fuligni, F., Etebari, M., Tabanelli, V., Laginestra, M. A., Pileri, A., Motta, G., Rossi, M., Agostinelli, C., Sabattini, E., Pimpinelli, N., Truni, M., Falini, B., Cerroni, L., Talarico, G., Piccioni, R., Amente, S., Indio, V., Tarantino, G., Brundu, F., Paulli, M., Berti, E., Facchetti, F., Dellino, G. I., Bertolini, F., Tripodo, C., Rabadan, R., Pileri, S. A. |
| سنة النشر: | 2019 |
| المجموعة: | IRIS UNIPV (Università degli studi di Pavia) |
| مصطلحات موضوعية: | Aged, Animal, Azacitidine, Cell Line, Tumor, Decitabine, Epigenesis, Genetic, Female, Gene Expression Regulation, Neoplastic, Human, Male, Mice, Inbred NOD, SCID, Middle Aged, Xenograft Model Antitumor Assay, Hematologic Neoplasm, Myeloproliferative Disorder, Neoplasm Protein, Skin Neoplasms |
| الوصف: | Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and aggressive hematologic malignancy for which there is still no effective B therapy. In order to identify genetic alterations useful for a new treatment design, we used whole-exome sequencing to analyze 14 BPDCN patients and the patient-derived CAL-1 cell line. The functional enrichment analysis of mutational data reported the epigenetic regulatory program to be the most significantly undermined (P<0.0001). In particular, twenty-five epigenetic modifiers were found mutated (e.g. ASXL1, TET2, SUZ12, ARID1A, PHF2, CHD8); ASXL1 was the most frequently affected (28.6% of cases). To evaluate the impact of the identified epigenetic mutations at the gene-expression and Histone H3 lysine 27 trimethylation/acetylation levels, we performed additional RNA and pathology tissue-chromatin immunoprecipitation sequencing experiments. The patients displayed enrichment in gene signatures regulated by methylation and modifiable by decitabine administration, shared common H3K27-acetylated regions, and had a set of cell-cycle genes aberrantly up-regulated and marked by promoter acetylation. Collectively, the integration of sequencing data showed the potential of a therapy based on epigenetic agents. Through the adoption of a preclinical BPDCN mouse model, established by CAL-1 cell line xenografting, we demonstrated the efficacy of the combination of the epigenetic drugs 5’-azacytidine and decitabine in controlling disease progression in vivo. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/30381297; info:eu-repo/semantics/altIdentifier/wos/WOS:000462841300029; volume:104; issue:4; firstpage:729; lastpage:737; numberofpages:9; journal:HAEMATOLOGICA; http://hdl.handle.net/11571/1373743Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85064014442 |
| DOI: | 10.3324/haematol.2018.202093 |
| الإتاحة: | https://doi.org/10.3324/haematol.2018.202093Test http://hdl.handle.net/11571/1373743Test |
| رقم الانضمام: | edsbas.67F8C942 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3324/haematol.2018.202093 |
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