دورية أكاديمية
Hypoglycemia, hypoxia, and ischemia in a corticostriatal slice preparation: electrophysiologic changes and ascorbyl radical formation
| العنوان: | Hypoglycemia, hypoxia, and ischemia in a corticostriatal slice preparation: electrophysiologic changes and ascorbyl radical formation |
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| المؤلفون: | PEDERSEN, JENS ZACHO, BERNARDI, GIORGIO, CENTONZE, DIEGO, PISANI, ANTONIO, ROSSI, LUISA, ROTILIO, GIUSEPPE, CALABRESI, PAOLO |
| المساهمون: | Pedersen, JENS ZACHO, Bernardi, Giorgio, Centonze, Diego, Pisani, Antonio, Rossi, Luisa, Rotilio, Giuseppe, Calabresi, Paolo |
| سنة النشر: | 1998 |
| المجموعة: | IRIS UNIPV (Università degli studi di Pavia) |
| مصطلحات موضوعية: | Corpus Striatum, Male, Free Radical, Hypoglycemia, Dehydroascorbic Acid, Rat, Wistar, Electron Spin Resonance Spectroscopy, Animal, Anoxia, Electric Stimulation, Ischemic Attack, Transient, L-Lactate Dehydrogenase |
| الوصف: | Experimental and clinical data suggest that oxygen and/or glucose deprivation alters electrical transmission in the brain and generates free radicals, which may mediate neuronal death. We have analyzed the effects of oxygen and/or glucose deprivation on both excitatory transmission, by measuring field potential amplitude, and free radical production, by using electron spin resonance (ESR) spectroscopy, in a corticostriatal slice preparation. Combined oxygen and/or glucose deprivation (ischemia) lasting 10 to 20 minutes induced a long-term depression of field potential amplitude. The ascorbyl radical could only be detected in brain slices during the reperfusion-phase after 30 minutes of ischemia. It appeared in the early minutes after the washout of ischemic medium and remained stable throughout the reperfusion phase. This radical was never detected in the external medium. Ischemia induced only a slight, but progressive, release of lactate dehydrogenase (LDH) into the external medium during the reperfusion phase. In contrast, exposure of slices to hypoxia or hypoglycemia alone resulted in transient depression of field potential amplitude, and no generation of ascorbyl radicals was observed on reperfusion. We propose that the long-lasting loss of electrical signals is the early sign of neuronal damage during ischemia. On the other hand, ascorbyl radical formation may be considered an indicator of neuronal injury after prolonged energy deprivation. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/PMID: 9701347; info:eu-repo/semantics/altIdentifier/wos/WOS:000075122800006; volume:18; issue:8; firstpage:868; lastpage:875; numberofpages:8; journal:JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM; http://hdl.handle.net/11571/1352615Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0031826377 |
| DOI: | 10.1097/00004647-199808000-00006 |
| DOI: | 10.1097/00004647-199808000-00006?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub++0pubmed& |
| الإتاحة: | https://doi.org/10.1097/00004647-199808000-00006Test http://hdl.handle.net/11571/1352615Test |
| رقم الانضمام: | edsbas.3052C600 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1097/00004647-199808000-00006 |
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