دورية أكاديمية
Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids
| العنوان: | Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids |
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| المؤلفون: | Kenney, Mary, Waters, Ryan A., Rieder, Elizabeth, Pega, Juan Franco, Perez Filgueira, Daniel Mariano, Golde, William T. |
| المصدر: | Journal of immunological methods 450 : 1-9. (November 2017) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2017 |
| المجموعة: | Inta Digital (ID - Instituto Nacional de Tecnología Agropecuaria) |
| مصطلحات موضوعية: | Virus Fiebre Aftosa, Respuesta Inmunológica, ELISA, Citometría de Flujo (Células), Aphthovirus, Immune Response, Flow Cytometry, Foot and Mouth Disease Virus, Biotinylation, Biotinilación |
| الوصف: | Analysis of the immune response to infection of livestock by foot-and-mouth disease virus (FMDV) is most often reported as the serum antibody response to the virus. While measurement of neutralizing antibody has been sensitive and specific, measurements of the quality of the antibody response are less robust. Determining the immunoglobulin (Ig) isotype of the serum antibody response provides a deeper understanding of the biology of the response and more sensitive methods for these assays will facilitate analyses of B cell mediated immunity. We tested the hypothesis that using the virus as the molecular probe could be achieved by adding tags to the surface of the FMDV capsid, and that would enhance sensitivity in assays for anti-FMDV antibody responses. The use of a FLAG-tagged virus in these assays failed to yield improvement whereas chemically biotinylating the virus capsid resulted in significant enhancement of the signal. Here we describe methods using biotinylated virus for measuring anti-viral antibody in serum and antibody secreting cells (ASCs) in blood that are sensitive and specific. Finally, we describe using the biotinylated virus in flow cytometry where such assays should greatly enhance the analysis of anti-virus antibody producing B cells, allowing the investigator to focus on only the FMDV specific B cells when analyzing the development of the B cell response to either infection or vaccination. ; Instituto de Virología ; Fil: Kenney, Mary. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos ; Fil: Waters, Ryan A. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos ; Fil: Rieder, Elizabeth. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos ; Fil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0022-1759 |
| العلاقة: | http://hdl.handle.net/20.500.12123/4444Test; https://www.sciencedirect.com/science/article/pii/S0022175917302144?via%3DihubTest; https://doi.org/10.1016/j.jim.2017.07.001Test |
| DOI: | 10.1016/j.jim.2017.07.001 |
| الإتاحة: | https://doi.org/20.500.12123/4444Test https://doi.org/10.1016/j.jim.2017.07.001Test https://hdl.handle.net/20.500.12123/4444Test https://www.sciencedirect.com/science/article/pii/S0022175917302144?via%3DihubTest |
| حقوق: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.29809F87 |
| قاعدة البيانات: | BASE |
| تدمد: | 00221759 |
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| DOI: | 10.1016/j.jim.2017.07.001 |