دورية أكاديمية
Characterization of the two component regulatory system PhoPR in Mycobacterium bovis
| العنوان: | Characterization of the two component regulatory system PhoPR in Mycobacterium bovis |
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| المؤلفون: | Garcia, Elizabeth Andrea, Blanco, Federico Carlos, Bigi, María Mercedes, Vazquez, Cristina Lourdes, Forrellad, Marina Andrea, Rocha, Rosana Valeria, Golby, Paul, Soria, Marcelo Abel, Bigi, Fabiana |
| المصدر: | Veterinary microbiology 222 : 30-38. (August 2018) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2018 |
| المجموعة: | Inta Digital (ID - Instituto Nacional de Tecnología Agropecuaria) |
| مصطلحات موضوعية: | Mycobacterium Bovis, Expresión Génica, Components, Gene Expression, PhoPR |
| الوصف: | Mycobacterium bovis is the causative agent of bovine tuberculosis and is a member of Mycobacterium tuberculosis complex, which causes tuberculosis in a number of mammals including humans. Previous studies have shown that the genes encoding the two-component system PhoPR, which regulates several genes involved in the virulence of M. tuberculosis, are polymorphic in M. bovis, when compared to M. tuberculosis, which results in a dysfunctional two-component system. In this study we investigated the role of PhoPR in two M. bovis strains with differing degrees of virulence. We found that the deletion of phoP in an M. bovis isolate reduced its capacity of inducing phagosomal arrest in bovine macrophages. By gene expression analysis, we demonstrated that, in both M. bovis strains, PhoP regulates the expression of a putative lipid desaturase Mb1404-Mb1405, a protein involved in redox stress AhpC, the sulfolipid transporter Mmpl8 and the secreted antigen ESAT-6. Furthermore, the lack of PhoP increased the sensitivity to acidic stress and alteration of the biofilm/pellicle formation of M. bovis. Both these phenotypes are connected to bacterial redox homeostasis. Therefore, the results of this study suggest a role of PhoPR in M. bovis to be linked to the mechanisms that mycobacteria display to maintain their redox balance. ; Instituto de Biotecnología ; Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía, Cátedra de Microbiología Agrícola; Argentina. . Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-0-378-11351-9 0-378-11351-8 |
| تدمد: | 0378-1135 |
| العلاقة: | http://hdl.handle.net/20.500.12123/4531Test; https://www.sciencedirect.com/science/article/pii/S0378113518300725?via%3DihubTest; https://doi.org/10.1016/j.vetmic.2018.06.016Test |
| DOI: | 10.1016/j.vetmic.2018.06.016 |
| الإتاحة: | https://doi.org/20.500.12123/4531Test https://doi.org/10.1016/j.vetmic.2018.06.016Test https://hdl.handle.net/20.500.12123/4531Test https://www.sciencedirect.com/science/article/pii/S0378113518300725?via%3DihubTest |
| حقوق: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.176997F8 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780378113519 0378113518 |
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| تدمد: | 03781135 |
| DOI: | 10.1016/j.vetmic.2018.06.016 |