دورية أكاديمية
Recombinant occlusion bodies of baculovirus as carriers of a nonstructural protein of foot-and-mouth disease virus
العنوان: | Recombinant occlusion bodies of baculovirus as carriers of a nonstructural protein of foot-and-mouth disease virus |
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المؤلفون: | Diez, Michay, Trotta, Myrian Vanesa, Alfonso, Victoria, Taboga, Oscar Alberto, Lopez, Maria Gabriela |
المصدر: | 3 Biotech 8 : 457. (November 2018) |
بيانات النشر: | Springer |
سنة النشر: | 2018 |
المجموعة: | Inta Digital (ID - Instituto Nacional de Tecnología Agropecuaria) |
مصطلحات موضوعية: | Baculovirus, Virus Fiebre Aftosa, Aphthovirus, ELISA, Occlusion Bodies, Foot and Mouth Disease Virus, Polyhedrin, FMDV, DIVA Diagnostic, Cuerpos de Oclusión |
الوصف: | Here, we developed a diagnostic ELISA for foot-and-mouth disease using recombinant occlusion bodies (rOBs) of baculovirus. We fused Δ3AB1–3, a polypeptide derived from non-structural proteins of foot-and-mouth disease virus, to polyhedrin (POLH), the major constituent of OBs, under polh promoter. To further assess the most convenient strategy to improve yields, we designed two recombinant baculoviruses, vPOLH and vPOLHE44G. These carried the sequence of the fusion protein POLH-Δ3AB1–3 with an additional copy in cis of polh or polhE44G, respectively, under p10 promoter. Our results show that both viruses expressed POLH-Δ3AB1–3, which was detected by western blot in purified rOBs with anti-POLH and anti-3AB1 antibodies. We also found that vPOLHE44G produced larger polyhedra and a significant increase of antigen yield (p < 0.01). Furthermore, the chimeric protein POLH-Δ3AB1–3 was recognized by sera from experimentally infected animals, showing that translational fusion to POLH does not alter the antigenicity of Δ3AB1–3. Finally, the rOBs were successfully used in an ELISA test to differentiate infected from vaccinated animals. Taken together, these results demonstrate the great potential of rOBs to develop diagnostic schemes adaptable to animal infectious diseases. ; Instituto de Biotecnología ; Fil: Diez, Michay. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Trotta, Myrian Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina ; Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ... |
نوع الوثيقة: | article in journal/newspaper |
وصف الملف: | application/pdf |
اللغة: | English |
تدمد: | 2190-572X |
العلاقة: | http://hdl.handle.net/20.500.12123/4459Test; 2190-5738 (Online); https://doi.org/10.1007/s13205-018-1482-xTest |
DOI: | 10.1007/s13205-018-1482-x |
الإتاحة: | https://doi.org/20.500.12123/4459Test https://doi.org/10.1007/s13205-018-1482-xTest https://hdl.handle.net/20.500.12123/4459Test |
حقوق: | info:eu-repo/semantics/restrictedAccess |
رقم الانضمام: | edsbas.4E2AD27B |
قاعدة البيانات: | BASE |
تدمد: | 2190572X |
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DOI: | 10.1007/s13205-018-1482-x |