دورية أكاديمية
Phenotypic and Genotypic Characteristics of Members of the Genus Streptobacillus
العنوان: | Phenotypic and Genotypic Characteristics of Members of the Genus Streptobacillus |
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المؤلفون: | Eisenberg, Tobias, Nicklas, Werner, Mauder, Norman, Rau, Joerg, Contzen, Matthias, Semmler, Torsten, Hofmann, Nicola, Aledelbi, Khayrieh, Ewers, Christa |
المصدر: | PLoS ONE 10 (2015), Nr. 8 |
بيانات النشر: | Public Library Science |
سنة النشر: | 2015 |
المجموعة: | Institutional Repository of Leibniz Universität Hannover |
مصطلحات موضوعية: | rat-bite fever, polymerase-chain-reaction, complete genome sequence, hongkongensis sp nov, fusobacterium-nucleatum, infrared-spectroscopy, phylogenetic analysis, fourier-transform, ribosomal-rna, moniliformis, ddc:610 |
الوصف: | The genus Streptobacillus (S.) remained monotypic for almost 90 years until two new species were recently described. The type species, S. moniliformis, is one of the two etiological agents of rat bite fever, an under-diagnosed, worldwide occurring zoonosis. In a polyphasic approach field isolates and reference strains of S. moniliformis, S. hongkongensis, S. felis as well as divergent isolates were characterized by comparison of molecular data (n = 29) and from the majority also by their physiological as well as proteomic properties (n = 22). Based on growth-independent physiological profiling using VITEK2-compact, API ZYM and the Micronaut system fastidious growth-related difficulties could be overcome and streptobacilli could definitively be typed despite generally few differences. While differing in their isolation sites and dates, S. moniliformis isolates were found to possess almost identical spectra in matrix-assisted laser desorption ionization-time of flight mass spectrometry and Fourier transform infrared spectroscopy. Spectroscopic methods facilitated differentiation of S. moniliformis, S. hongkongensis and S. felis as well as one divergent isolate. Sequencing of 16S rRNA gene as well as functional genes groEL, recA and gyrB revealed only little intraspecific variability, but generally proved suitable for interspecies discrimination between all three taxa and two groups of divergent isolates. ; Hessian Ministry for the Environment, Climate Change, Agriculture and Consumer Protection (HMUKLV) ; Hessian Ministry for the Environment, Climate Change, Agriculture and Consumer Protection (HMUKLV) |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | English |
العلاقة: | ESSN:1932-6203; http://dx.doi.org/10.15488/272Test; http://www.repo.uni-hannover.de/handle/123456789/294Test |
DOI: | 10.15488/272 |
الإتاحة: | https://doi.org/10.15488/272Test https://doi.org/10.1371/journal.pone.0134312Test http://www.repo.uni-hannover.de/handle/123456789/294Test |
حقوق: | CC BY 4.0 Unported ; http://creativecommons.org/licenses/by/4.0Test/ ; frei zugänglich |
رقم الانضمام: | edsbas.99F0D4A2 |
قاعدة البيانات: | BASE |
DOI: | 10.15488/272 |
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