دورية أكاديمية
A process independent of the anaphase-promoting complex contributes to instability of the yeast S phase cyclin Clb5
| العنوان: | A process independent of the anaphase-promoting complex contributes to instability of the yeast S phase cyclin Clb5 |
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| المؤلفون: | Sari, Fatih, Braus, Gerhard H., Irniger, Stefan |
| المصدر: | Journal of Biological Chemistry, The (JBC) 282 (2007), Nr. 36 ; Journal of Biological Chemistry, The (JBC) |
| بيانات النشر: | ASBMB Publications |
| سنة النشر: | 2007 |
| المجموعة: | Institutional Repository of Leibniz Universität Hannover |
| مصطلحات موضوعية: | Cyclin B, Cyclins, G1 Phase, Half-Life, Proteasome Endopeptidase Complex, ddc:570, ddc:540 |
| الوصف: | Proteolytic destruction of many cyclins is induced by a multi-subunit ubiquitin ligase termed the anaphase promoting complex/ cyclosome (APC/C). In the budding yeast Saccharomyces cerevisiae, the S phase cyclin Clb5 and the mitotic cyclins Clb1-4 are known as substrates of this complex. The relevance of APC/C in proteolysis of Clb5 is still under debate. Importantly, a deletion of the Clb5 destruction box has little influence on cell cycle progression. To understand Clb5 degradation in more detail, we applied in vivo pulse labeling to determine the half-life of Clb5 at different cell cycle stages and in the presence or absence of APC/C activity. Clb5 is significantly unstable, with a half-life of ∼8-10 min, at cell cycle periods when APC/C is inactive and in mutants impaired in APC/C function. A Clb5 version lacking its cyclin destruction box is similarly unstable. The half-life of Clb5 is further decreased in a destruction box-dependent manner to 3-5 min in mitotic or G 1 cells with active APC/C. Clb5 instability is highly dependent on the function of the proteasome. We conclude that Clb5 proteolysis involves two different modes for targeting of Clb5 to the proteasome, an APC/C-dependent and an APC/C-independent mechanism. These different modes apparently have overlapping functions in restricting Clb5 levels in a normal cell cycle, but APC/C function is essential in the presence of abnormally high Clb5 levels. © 2007 by The American Society for Biochemistry and Molecular Biology, Inc. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0021-9258 |
| العلاقة: | ESSN:1083-351X; http://dx.doi.org/10.15488/16156Test; https://www.repo.uni-hannover.de/handle/123456789/16283Test |
| DOI: | 10.15488/16156 |
| الإتاحة: | https://doi.org/10.15488/16156Test https://doi.org/10.1074/jbc.m703744200Test https://www.repo.uni-hannover.de/handle/123456789/16283Test |
| حقوق: | CC BY 4.0 Unported ; https://creativecommons.org/licenses/by/4.0Test ; frei zugänglich |
| رقم الانضمام: | edsbas.EF578870 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00219258 |
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| DOI: | 10.15488/16156 |