دورية أكاديمية
Proof‑of‑concept study to quantify changes in intestinal loads of KPC-producing Klebsiella pneumoniae in colonised patients following selective digestive decontamination with oral gentamicin
| العنوان: | Proof‑of‑concept study to quantify changes in intestinal loads of KPC-producing Klebsiella pneumoniae in colonised patients following selective digestive decontamination with oral gentamicin |
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| المؤلفون: | Pérez Nadales, Elena, Natera, Alejandra M., Recio Rufián, Manuel, Guzmán Puche, Julia, Cano, Ángela, Frutos Adame, Azahara, Castón, Juan José, Elías-López, Cristina, López Cerero, Lorena, Torre-Cisneros, Julian |
| المساهمون: | Universidad de Sevilla. Departamento de Microbiología, Agencia Estatal de Investigación. España, European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER), Ministerio de Ciencia, Innovación y Universidades (MICINN). España |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2022 |
| المجموعة: | idUS - Deposito de Investigación Universidad de Sevilla |
| مصطلحات موضوعية: | Intestinal colonisation, Selective digestive decontamination, Antimicrobial resistance, KPC-producing Klebsiella pneumoniae, Bacterial load |
| الوصف: | Objectives To monitor quantitatively the extent of intestinal colonisation by KPC-producing Klebsiella pneumoniae (KPC-Kp) in colonised patients who receive selective digestive decontamination (SDD) with oral gentamicin. Methods We developed a real-time quantitative PCR (qPCR) method for determination of the relative load of blaKPC (RLKPC) within the gut microbiota. Clinical validation was performed using a culture method as the gold standard and receiver operating curve (ROC) analysis. Fifteen patients were observationally and prospectively followed for one year. Clinical, microbiological variables and rectal swab samples were collected at 0 (baseline), 14 and 30 days and monthly thereafter. Results Clinical validation performed on 111 rectal swab samples demonstrated that the PCR method detected 17% more positives than the culture method. ROC curve analysis documented excellent agreement between both methods (area under the curve, 0.96; 95% confidence interval 0.93–0.99). The RLKPC decreased in 6/15 (40%) and 7/12 (58.3%) patients on days 14 and 30, respectively. Persistent eradication was observed in 2/12 (16.7%), 3/9 (33.3%), 4/8 (50%) and 7/8 (87.5%) patients at 1, 3, 6 and 12 months, respectively, with a median time of 150 days (range 30–270) to persistent eradication. Gentamicin-resistant KPC-Kp isolates were identified in 4/15 (26.7%) patients. The rates of infections (57.1% vs. 12.5%, P = 0.119) and deaths (71.4% vs. 0%, P = 0.007) were higher among patients with high baseline RLKPC. Conclusion Following SDD, a rapid reduction on intestinal load is observed when the colonising KPC-Kp isolate is susceptible to gentamicin; however, persistent eradication at the end of SDD is low. Intestinal carriage of KPC-Kp persists after three months in about one third of patients. ; Agencia Española de Investigación and Fondo Europeo de Desarrollo Regional (FEDER) FIS PI16/01631 ; Ministerio de Ciencia, Innovación y Universidades RD16/0016/0008 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | Journal of Global Antimicrobial Resistance, 30, 16-22.; FIS PI16/01631; RD16/0016/0008; https://dx.doi.org/10.1016/j.jgar.2022.04.010Test; https://idus.us.es/handle//11441/138121Test |
| الإتاحة: | https://doi.org/10.1016/j.jgar.2022.04.010Test https://idus.us.es/handle//11441/138121Test |
| حقوق: | Atribución 4.0 Internacional ; http://creativecommons.org/licenses/by/4.0Test/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.7DA48F72 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |