دورية أكاديمية
Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source
| العنوان: | Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source |
|---|---|
| المؤلفون: | Zou, X., State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P. R. China, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, P. R. China, Chen, C.-F., Hang, H.-F., Chu, J., juchu@ecust.edu.cn, Zhuang, Y.-P., Zhang, S.-L. |
| المصدر: | Chemical & Biochemical Engineering Quarterly (cabeq@pbf.hr); Vol.24 No.1 |
| بيانات النشر: | Croatian Society of Chemical Engineers; kui@zg.t-com.hr |
| سنة النشر: | 2010 |
| المجموعة: | Hrčak - Portal of scientific journals of Croatia / Portal znanstvenih časopisa Republike Hrvatske |
| مصطلحات موضوعية: | Biological nitrogen, erythromycin production, fed-batch strategy, medium optimization, response surface methodology, Saccharopolyspora erythraea |
| الوصف: | A novel source of biological nitrogen produced by multi-strain fermentation was developed for erythromycin production.The nitrogen composition of corn steep liquor, biological nitrogen and soybean flour was optimized by using Response surface methodology (RSM) firstly. A Box-Behnken design was used to estimate the optimum nitrogen composition for the production of erythromycin as follows: corn steep liquor: γ =5.1 g L-1, biological nitrogen γ = 5.96 g L-1 and soybean flour γ = 24.17 g L-1. Based on the optimum nitrogen source, the constant glucose fed-batch and pH control fed-batch strategies were used to further optimize the erythromycin production in 50 L stirred bioreactor, respectively.It was found that pH control could serve as an effective control strategy for erythromycin production, and the maximum erythromycin production was 8528 U mL-1 at 190 h.This work demonstrates that the new medium formulation based on a cheap nitrogen source, biological nitrogen, is a potential alternative for economic erythromycin production on a large scale. |
| نوع الوثيقة: | text |
| وصف الملف: | |
| اللغة: | English |
| العلاقة: | http://hrcak.srce.hr/49490Test; http://hrcak.srce.hr/file/76178Test |
| الإتاحة: | http://hrcak.srce.hr/49490Test http://hrcak.srce.hr/file/76178Test |
| رقم الانضمام: | edsbas.4C4BA693 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |