دورية أكاديمية
Dokaz sudanskih izolata serološke skupine virusa bolesti plavog jezika pomoću lančane reakcije polimerazom (PCR). ; PCR detection of Sudanese isolates of bluetongue virus serogroup.
| العنوان: | Dokaz sudanskih izolata serološke skupine virusa bolesti plavog jezika pomoću lančane reakcije polimerazom (PCR). ; PCR detection of Sudanese isolates of bluetongue virus serogroup. |
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| المؤلفون: | Abdalla, Mohammed, Department of Medicine, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Khartoum, Sudan, Suliman, Siham, Karrar, Abdelrahim, Idris, Salah, Central Veterinary Research Laboratories, Soba, Khartoum, Sudan, Aradaib, Imadeldin, aradaib@yahoo.com |
| المصدر: | Veterinary Archives (veterinarski.arhiv@vef.hr); Vol.73. No.3 |
| بيانات النشر: | Faculty of Veterinary Medicine University of Zagreb |
| سنة النشر: | 2003 |
| المجموعة: | Hrčak - Portal of scientific journals of Croatia / Portal znanstvenih časopisa Republike Hrvatske |
| مصطلحات موضوعية: | orbivirusi, virus bolesti plavog jezika, RT-PCR, Sudan, orbiviruses, bluetongue virus, reverse transcriptase-polymerase chain reaction |
| الوصف: | Lančana reakcija polimerazom uz reverznu transkripciju (RT-PCR) prethodno je razvijena za dokazivanje ribonukleinske kiseline sjevernoameričkih izolata virusa bolesti plavog jezika (BPJ) umnoženih u staničnoj kulturi te je ujedno rabljena i za dokazivanje sudanskih izolata virusa BPJ. Za RT-PCR amplifikaciju odabrana su dva para oligonukleotidnih početnica na genu koji kodira za nestrukturni protein 1 (NS1) virusa BPJ-17. Pomoću početnica dobiven je amplifikacijski proizvod od 826 parova baza (pb). Ovim RT-PCR postupkom dokazana je RNA sudanskih serotipova 4 i 16 umnoženih u staničnim kulturama. Amplifikacijski proizvod nije bio dokazan kad je metoda bila primijenjena za dokazivanje RNA usko srodnog serotipa 4 sudanskog orbivirusa epizootske hemoragijske bolesti i nukleinske kiseline izdvojene iz neinficiranih stanica Vero. Rezultati ovog istraživanja pokazuju da se ranije opisani RT-PCR za virus BPJ može koristiti za dokazivanje sudanskih izolata virusa BPJ i moguće drugih serotipova serološke skupine virusa BPJ s različitih kontinenata. ; The potential use of the recently reported reverse transcriptase (RT) polymerase chain reaction (RT-PCR)-based assay for detection of North American isolates of bluetongue virus (BTV) ribonucleic acid in cell culture was evaluated for detection of Sudanese isolates of BTV. Two pairs of oligoribonucleotide primers, selected from non-structural protein 1 (NS1) gene of BTV-17, were used for RT-PCR amplification. The BTV RT-PCR produced an 826 base pair (bp) amplification product. RNA from Sudanese BTV serotypes 4 and 16, propagated in cell cultures, were detected by this RT-PCR-based assay. Amplification product was not detected when the nested BTV RT-PCR based assay was applied to RNA from closely related Sudanese Orbivirus, epizootic hemorrhagic disease virus (EHDV) serotype 4 and total nucleic acid extracts from uninfected Vero cells. The results of this study indicated that our previously described BTV RT-PCR assay could be used for detection of the Sudanese BTV isolates and ... |
| نوع الوثيقة: | text |
| وصف الملف: | |
| اللغة: | English |
| العلاقة: | http://hrcak.srce.hr/74727Test; http://hrcak.srce.hr/file/110936Test |
| الإتاحة: | http://hrcak.srce.hr/74727Test http://hrcak.srce.hr/file/110936Test |
| حقوق: | Full-text papers can be used for personal or educational purposes only. Authors' and publisher's copyrights must be acknowledged. |
| رقم الانضمام: | edsbas.8657F6AF |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |