دورية أكاديمية
RANKL regulates differentiation of microfold cells in mouse nasopharynx-associated lymphoid tissue (NALT)
| العنوان: | RANKL regulates differentiation of microfold cells in mouse nasopharynx-associated lymphoid tissue (NALT) |
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| المؤلفون: | Mutoh, Mami, Kimura, Shunsuke, Takahashi-Iwanaga, Hiromi, Hisamoto, Meri, Iwanaga, Toshihiko, Iida, Junichiro |
| بيانات النشر: | Springer |
| المجموعة: | Hokkaido University Collection of Scholarly and Academic Papers (HUSCAP) / 北海道大学学術成果コレクション |
| مصطلحات موضوعية: | Microfold cells (M cells), Follicle-associated epithelium, Nasopharynx-associated lymphoid tissue (NALT), Glycoprotein 2 (GP2), Receptor activator of nuclear factor kappa-B ligand (RANKL) |
| الوقت: | 490 |
| الوصف: | Murine nasopharynx-associated lymphoid tissue (NALT), located at the base of the nasal cavity, serves as a major site for the induction of mucosal immune responses against airway antigens. The follicle-associated epithelium (FAE) covering the luminal surface of NALT is characterized by the presence of microfold cells (M cells), which take up and transport luminal antigens to lymphocytes. Glycoprotein 2 (GP2) has recently been identified as a reliable marker for M cells in Peyer's patches of the intestine. However, the expression of GP2 and other functional molecules in the M cells of NALT has not yet been examined. We have immunohistochemically detected GP2-expressing cells in the FAE of NALT and the simultaneous expression of other intestinal M-cell markers, namely Tnfaip2, CCL9, and Spi-B. These cells have been further identified as M cells because of their higher uptake capacity of luminal microbeads. Electron microscopic observations have shown that GP2-expressing cells on the FAE display morphological features typical of M cells: they possess short microvilli and microfolds on the luminal surface and are closely associated with intraepithelial lymphocytes. We have also found that the receptor activator of nuclear factor kappa-B ligand (RANKL) is expressed by stromal cells underneath the FAE, which provides its receptor RANK. The administration of RANKL markedly increases the number of GP2+Tnfaip2+ cells on the NALT FAE and that of intestinal M cells. These results suggest that GP2+Tnfaip2+ cells in NALT are equivalent to intestinal M cells, and that RANKL-RANK signaling induces their differentiation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | http://hdl.handle.net/2115/64947Test; Cell and Tissue Research, 364(1): 175-184; http://dx.doi.org/10.1007/s00441-015-2309-2Test |
| DOI: | 10.1007/s00441-015-2309-2 |
| الإتاحة: | https://doi.org/10.1007/s00441-015-2309-2Test http://hdl.handle.net/2115/64947Test |
| حقوق: | The final publication is available at link.springer.com |
| رقم الانضمام: | edsbas.B1BE1E20 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00441-015-2309-2 |
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