دورية أكاديمية
An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A
| العنوان: | An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A |
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| المؤلفون: | Mazaafrianto, Donny Nugraha, Ishida, Akihiko, Maeki, Masatoshi, Tani, Hirofumi, Tokeshi, Manabu |
| بيانات النشر: | Japan Society for Analytical Chemistry |
| المجموعة: | Hokkaido University Collection of Scholarly and Academic Papers (HUSCAP) / 北海道大学学術成果コレクション |
| مصطلحات موضوعية: | Dithiol, microfabricated device, electrochemical detection, aptamer, methylene blue, ochratoxin A, signal-on |
| الوقت: | 433 |
| الوصف: | In this study, we developed an electrochemical sensor for ochratoxin A (OTA) by using an aptamer having a dithiol-based anchor, which exhibited higher stability on a gold electrode than a monothiol-based aptamer because of its two anchors. The sensor was also based on a signal-on scheme that produces a signal current resulting from structure-switching of the aptamer upon interaction with OTA. For simple fabrication of this sensor, the non-covalent interaction of methylene blue with the aptamer was also employed as an electrochemical indicator. In this study, the performance of the sensor, including the dissociation constant of the aptamer-OTA complex, was characterized. The proposed sensor exhibited high reproducibility and enough sensitivity to detect the minimum amount of OTA required for the analysis of real food samples with a limit of detection of 113 pM. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | http://hdl.handle.net/2115/76500Test; Analytical sciences, 35(11): 1221-1226; http://dx.doi.org/10.2116/analsci.19P240Test |
| DOI: | 10.2116/analsci.19P240 |
| الإتاحة: | https://doi.org/10.2116/analsci.19P240Test http://hdl.handle.net/2115/76500Test |
| رقم الانضمام: | edsbas.D5C556D8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.2116/analsci.19P240 |
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