دورية أكاديمية
Hyporesponsiveness following booster immunization with bacterial polysaccharides is caused by apoptosis of memory B cells.
| العنوان: | Hyporesponsiveness following booster immunization with bacterial polysaccharides is caused by apoptosis of memory B cells. |
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| المؤلفون: | Brynjolfsson, Siggeir F, Henneken, Maren, Bjarnarson, Stefania P, Mori, Elena, Del Giudice, Giuseppe, Jonsdottir, Ingileif |
| المساهمون: | Department of Immunology, Landspitali, The National University Hospital of Iceland, Reykjavik, Iceland. |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2012 |
| المجموعة: | Hirsla - Landspítali University Hospital research archive |
| مصطلحات موضوعية: | Adjuvants, Immunologic, Animals, Newborn, Annexin A5, Antigens, CD45, Apoptosis, B-Lymphocytes, Bone Marrow, Female, Flow Cytometry, Immunization, Secondary, Immunologic Memory, Immunophenotyping, Meningococcal Vaccines, Mice, Oligodeoxyribonucleotides, Polysaccharides, Bacterial, Spleen, Syndecan-1, Vaccines, Conjugate |
| الوصف: | To access publisher full text version of this article. Please click on the hyperlink in Additional Links field. ; Repeated immunizations with polysaccharide (PS) vaccines cause hyporesponsiveness through undefined mechanisms. We assessed the effects of a PS booster on immune responses, frequency, and survival of PS-specific B-cell subpopulations in spleen and bone marrow. Neonatal mice were primed with meningococcus serotype C (MenC) conjugate MenC-CRM(197)+CpG1826, boosted with MenC-CRM(197), MenC-PS, or saline; subsequently, bromodeoxyuridine (BrdU) was injected daily intraperitoneally. MenC-PS-specific cells were labeled with fluorescent MenC-PS and phenotyped by flow cytometry. After MenC-PS booster, proliferating (BrdU(+)) MenC-PS-specific naive B cells (CD138(-)/B220(+); P = .0003) and plasma cells (CD138(+)/B220(-); P = .0002) in spleen were fewer than after saline booster. BrdU(+) MenC-PS-specific plasma cells were also reduced in bone marrow (P = .0308). Compared to saline, MenC-PS booster reduced BrdU(+) IgG(+) MenC-PS-specific B cells in spleen (P = .0002). Twelve hours after the MenC-PS booster, an increased frequency of apoptotic (AnnexinV(+)) MenC-PS-specific B cells in spleen was observed compared with MenC-CRM(197) (P = .0286) or saline (P = .001) boosters. We demonstrated that the MenC-PS booster significantly reduced the frequency of newly activated MenC-PS-specific B cells-mostly switched IgG(+) memory cells-by driving them into apoptosis. It shows directly that apoptosis of PS-specific memory cells is the cause of PS-induced hyporesponsiveness. These results should be taken into account prior to consideration of the use of PS vaccines. ; University of Iceland, Landspitali University Hospital |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1537-6613 |
| العلاقة: | http://dx.doi.org/10.1093/infdis/jir750Test; J. Infect. Dis. 2012, 205(3):422-30; http://hdl.handle.net/2336/238397Test; Journal of infectious diseases |
| DOI: | 10.1093/infdis/jir750 |
| الإتاحة: | https://doi.org/10.1093/infdis/jir750Test http://hdl.handle.net/2336/238397Test |
| حقوق: | Archived with thanks to The Journal of infectious diseases ; Landspitali Access - LSH-aðgangur |
| رقم الانضمام: | edsbas.F4A6AA4 |
| قاعدة البيانات: | BASE |
| تدمد: | 15376613 |
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| DOI: | 10.1093/infdis/jir750 |