دورية أكاديمية
Establishment of a relationship between blastomere geometry and YAP localisation during compaction
| العنوان: | Establishment of a relationship between blastomere geometry and YAP localisation during compaction |
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| المؤلفون: | Royer, Christophe, Leonavicius, Karolis, Kip, Annemarie, Fortin, Deborah, Nandi, Kirtirupa, Vincent, Anna, Jones, Celine, Child, Tim, Coward, Kevin, Graham, Chris, Srinivas, Shankar |
| بيانات النشر: | The Company of Biologists Ltd |
| سنة النشر: | 2020 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | RESEARCH ARTICLE |
| الوصف: | Precise patterning within the three-dimensional context of tissues, organs and embryos implies that cells can sense their relative position. During preimplantation development, outside and inside cells rely on apicobasal polarity and the Hippo pathway to choose their fate. Despite recent findings suggesting that mechanosensing might be central to this process, the relationship between blastomere geometry (i.e. shape and position) and the Hippo pathway effector YAP remains unknown. We used a highly quantitative approach to analyse information on the geometry and YAP localisation of individual blastomeres of mouse and human embryos. We identified the proportion of exposed cell surface area as most closely correlating with the nuclear localisation of YAP. To test this relationship, we developed several hydrogel-based approaches to alter blastomere geometry in cultured embryos. Unbiased clustering analyses of blastomeres from such embryos revealed that this relationship emerged during compaction. Our results therefore pinpoint the time during early embryogenesis when cells acquire the ability to sense changes in geometry and provide a new framework for how cells might integrate signals from different membrane domains to assess their relative position within the embryo. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://dev.biologists.org/cgi/content/short/147/19/dev189449Test; http://dx.doi.org/10.1242/dev.189449Test |
| DOI: | 10.1242/dev.189449 |
| الإتاحة: | https://doi.org/10.1242/dev.189449Test http://dev.biologists.org/cgi/content/short/147/19/dev189449Test |
| حقوق: | Copyright (C) 2020, Company of Biologists |
| رقم الانضمام: | edsbas.965F72F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1242/dev.189449 |
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